Cyanogen bromide chromatography

Hapten density, and also the common positions where haptens are bound, can also be estimated by cyanogen bromide or enzymatic cleavage of the protein and either MALDI-MS or separation of the components by reversed-phase ion-pair chromatography and electrospray or electrospray time-of-flight (TOF) analysis. 

This method was used, for example, for the solid-phase immunoassay of thyroxine (affinity chromatography). Various activation methods (CDI, periodate, and cyanogen bromide procedures) were compared with each other for coupling antibodies to magnetizable cellulose/iron oxide solid-phase particles. 211]... 

March, S.C., Parikh, I., and Cuatrecasas, P. (1974) A simplified method for cyanogen bromide activation of agarose for affinity chromatography. Anal. Biochem. 60, 149-152. 

It should be mentioned here that another family of hydrocarbonaceous phases has been developed for the hydrophobic chromatography of biopolymers 49, 50). For the preparation of such stationary phases agarose beads are first activated with cyanogen bromide and subsequently reacted... 

Immunoaffinity chromatography cleanup has also been applied as an ideal and reliable strategy for residue analysis. Immunoaffinity columns prepared by coupling the antibodies to a cyanogen bromide-activated support were used to analyze avermectin BI residues in cattle tissues (359) and ivermectin in sheep serum (376). An immunoaffinity column prepared by an alternative activation/ coupling procedure with carbonyl diimidazole was also employed to analyze ivermectin residues in swine liver (361) since the earlier-reported methods did not work well in the analysis of this matrix. This recent work demonstrated the high specificity of tire antibody-mediated cleanup, but also showed that the immunoaffinity procedures could not always or completely eliminate matrix interference of samples. Therefore, application of additional cleanup steps before or after these procedures is often inevitable. 

Antibody affinity chromatography is employed to isolate antigen-specific antibodies. The most common affinity matrix for coupling of molecules is cyanogen bromide-activated... 

Cyanogen bromide is then separated by gas chromatography and selectively detected with an electron capture detector. 

Nota and Improta [813] determined cyanide in coke oven waste water by gas chromatography. The method is based on treatment of the sample with bromine and direct selective determination of the cyanogen bromide by gas solid chromatography using a BrCN selective electron capture detector. No preliminary treatment of the sample to remove interferences is necessary in this method, and in this sense it has distinct advantages over many of the earlier procedures. Bromine also oxidises thiocyanate to cyanogen bromide. Previous treatment of the sample with aqueous formaldehyde destroys thiocyanate and prevents its interference. 

Identification of SeMet after being separated from other amino acids by means of paper chromatography and electrophoresis is facilitated by spraying spots with H202 or preferably by exposure to cyanogen bromide (Shepherd and Huber, 1969). Pre-derivatization of SeMet through reaction with o-benzoquinone facilitates separation and identification in the presence of Met. Both SeMet and Met react with o-benzoquinone at pH 1 to form phenolic sulfonium- or selenonium derivatives whose UV absorption spectra differ. 

Zheng, O. and Wu, J. 1988. The determination of selenomethionine in selenium yeast by cyanogen bromide gas chromatography. Biomed. Chromatogr. 2, 258-259. 

Figure 7.6 Cyanogen bromide activation and coupling of a matrix for affinity chromatography.

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