Affinity chromatography for purification

Serpa G, Augusto EFP, Tamashiro WMSC, Ribeiro MB, Miranda EA, Bueno SMA (2005), Evaluation of immobilized metal membrane affinity chromatography for purification of IgGl monoclonal antibody, J. Chromatogr. B 816 259-268. 

Yang L, Jia L, Zou H, and Zhang Y. Immobilized iminodiacetic acid (IDA)-t)fpe Cu chelating membrane affinity chromatography for purification of bovine liver catalase. Biomed. Chromatogr. 1999 13 229-234. 

P. N. Srivastava and A. A. Farooqui. Heparin-Sepharose affinity chromatography for purification of bull seminal plasma hyaluronidase. Biochem. J. 755 531 (1979). 

Platis, D., and Labrou, N.E. (2008). Affinity chromatography for the purification of therapeutic proteins from transgenic maize using immobilized histamine. J. Sep. Sci. 31(4) 636-643. 

The availability of a great variety of group-specific adsorbents in prepacked columns makes possible the combination of FPLC and affinity chromatography for the separation and purification of proteins. 

Purification of synthetic proteins requires approaches that separates closely related byproducts from the correct molecule. Affinity chromatography for example, is not useful for synthetic peptides. Routinely used are chromatographic... 

Purification performance of metal chelate affinity chromatography for antibodies can be very attractive as very high purity levels in a single pass have been reported.191 Known contaminants of purified antibodies using metal chelate affinity chromatography include transferrin, and albumin traces. Eluted antibodies can also be contaminated by significant amounts of metal ions that must be removed for the following separation or before the final formulation is performed. 

The classical column procedures for the preparation of lysozyme are difficult to use with high viscosity solutions such as serum. A batch method was developed based on affinity chromatography using deami-nated chitin [82]. The method was found to give a one-step purification of nearly theoretical amounts in tissue homogenates tested. These included tissue homogenates from humans, primates, avian egg white and plants. The use of a chitin-coated cellulose affinity column for purification of lysozyme has also been described [83]. 

Wofsy, L and Burr, B (1969) The use of affinity chromatography for the specific purification of antibodies and antigens J Immunol. 103, 380-382... 

Zhou D, Zou H, Ni J, Wang H, Yang L, and Zhang Y. Membrane affinity chromatography for analysis and purification of biopolymers. Chromatographia 1999 50 23-27. 

A. S. Clairbois, D. Letourneur, D. Muller, and J. Joze-fonvicz. High-performance affinity chromatography for the purification of heparin-binding proteins from detergent-solubilized smooth muscle cell membranes, J. Chromatogr. B 706 55 (1998). 

The importance of polysaccharides as support media in the field of affinity chromatography continues, as numerous reviews have indicated. Quantitative uses of affinity chromatography in purification, functional characterization, determination of rate constants, and measurement of macromolecule-ligand equilibrium binding have been discussed. Improvement of affinity chromatographic separations may be achieved by use of mixed solvents at carefully controlled concentrations and at temperatures in the normal to sub-zero range. Results of a theoretical study of the concentration dependence of elution volume for particular ligands have been presented. ... 

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