Adsorption chelates inorganic complexes

The produced sulphuric acid facilitates the solution of Cu in waters and its migration in the form of sulphates or other compounds like organic and inorganic complexes, especially chelates. The migration of Cu in aquatic systems is hindered by the presence of CaC03, by adsorption on hydrous Fe-Mn-oxides, clay minerals and organic substances. In addition to this, the presence of H2S in extreme situations may reduce completely the aureole of waters enriched in Cu around the deposit. 

Type 3 metal complexes involve the physical interaction of a metal complex, chelates, or metal cluster with an organic polymer or inorganic high molecular weight compound. The preparation of type 3 compounds differs from those of type 1 and type 2, as they are ultimately achieved through the use of adsorption, deposition by evaporation, microencapsulation, and various other methods. 

Colorimetric assays are appropriate methods to investigate protein interactions with cationic polysaccharide materials. However, these assays cannot differentiate between different types of interactions, i.e. adsorption and absorption of proteins. Among these, the bicinchoninic acid assay (BCA) is widely used since it allows an easy determination of the total amount of adsorbed proteins on bulk materials." The assay is based on the reduction of Cu to Cu in a reductive environment in the presence of proteins. The BCA chelates Cu, forming a colored complex whose absorption band at 562 nm can be related to the amount of adsorbed proteins on the materials. Using this method, it has been demonstrated that the incorporation of inorganic materials such as hydroxyapatite (HAP) or nano-HAP into chitosan-based materials leads to the formation of protein-rich layers at the... 

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