Adoptive transfer system

Kristofferson A, Ahlstedt A, Pettersson E, Svaerd PO (1977) Antigens in penicillin allergy. I. A radioimmunoassay for detection of penicilloylated protein contaminants in penicillin preparations. Int Arch Allergy Appl Immunol 55 13 Lee WY, Sehon AH (1975) Suppression of reaginic antibody formation II. The use of adoptive transfer system for the study of immunological unresponsiveness. J Immunol 114 837... 

The regulations adopted by the California Department of Food and Agriculture requiring the use of closed-transfer systems went into effect in April 1977. At that time, several types of equipment were put into use, and a series of studies were undertaken to evaluate their effectiveness in protecting workers. 

There are reports of correlations of defective or deficient NKT cells in a number of autoimmune diseases in mice and humans (Sumida et al., 1995 Baxter et al., 1997, Wilson et al., 1998 Hies et al., 2000 Mieza et al., 1996 Zeng et al., 2000 Shi et al., 2001 Nagane et al., 2001). In humans, diabetes, systemic sclerosis, myasthenia gravis, and multiple sclerosis and in mice, a lupus model, are associated with NKT cell deficiencies. It was demonstrated that the adoptive transfer of NKT cells prevented diabetes in NOD mice. However, it is not clear whether the effectiveness of this treatment was actually due to the establishment of immune deviation of Tr cell-mediated aedve tolerance. 

So-called natural, or nonadaptive immunity also appears to have a prominent role in controlling the malignant phenotype of tumor cells. Both mice and humans with a low natural killer (NK) activity are more susceptible to develop metastases, whereas adoptive transfer of purified large granular lymphocytes (LGL a subset of cells closely associated with NK activity) to immunosuppressed rodents restored their resistance to metastasis (reviewed in White-side et al., 1998). LGL and Kupffer cells are involved in the defense system of the hver under various physiological and pathological conditions, hi a study with nude mice it was reported that only after... 

In the secondary popliteal lymph node assay, pretreated animals are re-exposed to the same chemical or to a metabolite in a dose that itself is incapable of stimulating naive T cells. A measured response to this low dose strongly indicates, but does not formally prove, that memory T cells are present. Proof for the formation of memory T cells can be obtained with the adoptive transfer popliteal lymph node assay in which purified T cells obtained from systemically treated mice are transferred to naive recipients that subsequently receive an injection into the paw of a non-sensitizing dose of the same chemical or a relevant metabolite. 

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