Adenosine triphosphatase properties

Nelson N, Nelson H and Packer E (1972) Partial resolution of enzymes catalyzing photophosphorylation Magnesium -adenosine triphosphatase properties of heat-activated coupling factor 1 from chloroplasts, J. Biol, Chem. 247, 6506-6510. 

For many years, niclosamide (Niclocide) was widely used to treat infestations of cestodes. Niclosamide is a chlorinated salicylamide that inhibits the production of energy derived from anaerobic metabolism. It may also have adenosine triphosphatase (ATPase) stimulating properties. Inhibition of anaerobic incorporation of inorganic phosphate into ATP is detrimental to the parasite. Niclosamide can uncouple oxidative phosphorylation in mammalian mitochondria, but this action requires dosages that are higher than those commonly used in treating worm infections. 

Johnston, I.A., Frearson, N. and Goldspink, G. (1973). The effects of environmental temperature on the properties of myofibrillar adenosine triphosphatase from various species of fish. Biochemical Journal 133,735-738. 

The nervous system is the main site of toxicity for DDT. Effects are observed on both the central nervous system (CNS) and peripherally. There is significant alteration of neuronal membrane enzymatic and electrophysiological properties. In particular, sodium channels are altered such that once activated they close slowly, prolonging the depolarization of the nerve by interfering with the active transport of Na ions out of the axon. Potassium channels are also affected. DDT specifically affects Na", K -adenosine triphosphatases (ATPases) and Ca " -AT-Pases, which inhibit repolarization of neurons. The membrane remains partially depolarized and is extremely sensitive to complete depolarization by very small stimuli. DDT also inhibits calmodulin that is necessary for Ca transport essential for the subsequent release of neurotransmitters. 

Another possibility deserving consideration is that some of the phytoestrogen effects may be attributable to properties that do not involve estrogen receptors, such as effects on enzymes, protein synthesis, cell proliferation, angiogenesis, calcium transport, Na /K adenosine triphosphatase, growth factor action, vascular smooth muscle cells, lipid oxidation, and cell differentiation. Some of these properties will be further discussed. 

Skubitz KM, Smith TW (1975) Determination of antibody-hapten association kinetics. A simplified experimental approach. J Immunol 114 1369-1374 Smith TW (1972) Ouabain-specific antibodies. Immunochemical properties and reversal of Na,K activated adenosine triphosphatase inhibition. J Clin Invest 51 1583-1593 Smith TW, Harber E (1973) Classical value of the radioimmunoassay of the digitalis glycosides. Pharmacol Rev 25 219-228... 

M.E. Pullman, H.S. Penefsky, A. Datta, and E. Racker, Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation. I. Purification and Properties of Soluble, Dinitrophe-nol-stimulated Adenosine Triphosphatase. J. Biol. Chem., 235,3322-3329,1960. 

Pullman, M.E., Penefsky, H.S., Datta, A., Racker, E. Partial resolution of the enzymes catalyzing oxidative phosphorylation I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase. J. biol. Chem. 235, 3322-3329 (1960)... 

Webster has also reported some preliminary investigations on the properties of the soluble protein formed in vitro. It seems to be composed of several electrophoretically distinct components, and also to contain adenosine triphosphatase activity. 

Kasamo K. Purification and properties of the plasma membrane H -translocating adenosine triphosphatase of Phaseolus munqo L. roots. Plant Physiol. 1986 80 818-824. Peterson GL. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 1977 83 346-356. 

Farron F (1970) Isolation and properties of a chloroplast coupling factor and heat-activated adenosine triphosphatase, Biochemistry 9, 3823-2828. 

Ohta S, Tsuboi M, Yoshida M and Kagawa Y (1980) Inter subunit interaction in proton translocating adenosine triphosphatase as revealed by hydrogen exchange kinetics. Biochemistry 19t 2160-2164. Pullman ME and Monroy GC (1963) A naturally occuring inhibitor of mitochondrial adenosine triphosphtase, J. Biol. Chem. 238, 3762-3769. Rott R and Nelson N (1981) Purification and immunological properties of proton ATPase complexes from yeast and rat liver mitochondria, J. Biol. Chem. 256, 9224-9228. 

V-Alkyl- and -aryl derivatives of chromone-2-carboxamide (which also have analgesic properties [195] (p. 106)) are described as exhibiting notable anticoagulant activity [11] but members of the same team found a low degree of antibacterial action in the chromone-2,3-lactone (110) and its 6-methyl, -bromo and -chloro derivatives [139]. The Clermont-Ferrand team recently reported that chromone-2-carbohydroxamic acid (sodium salt), when injected into the male rat, had no effect on the activities of the enzymes lactate dehydrogenase, transaminases and adenosine 5 -triphosphatase... 

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