ACQUITY UPLC

FIGURE 9.2 Comparison of Acquity UPLC column on HPLC and UPLC instruments. (Courtesy of Waters Corp.)... 

A Waters Acquity UPLC system with a cooling autosampler and column oven was used. The stationary phase was a Waters Acquity BEH C18 column (50 x 2.1 mm, 1.7 /.un particle size). The column was maintained at 40°C. The mobile phase consisted of water and acetonitrile, each containing 0.3% formic acid and was delivered at 0.35 mL/min in a gradient mode at 60% water from 0 to 1.5 min, linearly decreased to 10% water in 0.5 min, and then returned to 60% water. Sample vials were maintained at 4°C. 

Lercanidipine — Kalovidouris et al.57 applied UPLC-MS/MS to the determination of lercani-dipine in human plasma after oral administration of lercanidipine. A Waters Acquity UPLC system with cooling autosampler and column oven was coupled with a Waters BEH C18 column (50 x 2.1 mm, 1.7 jum). The mobile phase was composed of 70% acetonitrile in water containing 0.2% v/v formic acid, delivered at a flow of 0.30 mL/min. The column temperature was maintained at 40°C and sample vials at 5°C. 

FIGURE 5 (a) Peptide digest run on a 4.8-gm particle on a traditional HPLC system. Peak count is 70, and peak capacity is 143. (b) Peptide digest run on a 1.7-gm particle on the ACQUITY UPLC system. Peak count is 168, and peak capacity is 360. (Courtesy of Waters Corp.)... 

AT, FB, and their impurities Tablets Acquity UPLC BEH C18 column (1.7 urci, 2.1 mm x 100 mm) Gradient elution of acetonitrile ammonium acetate (pH 4.7 0.01 M) Minimum detection limit -was atng/1 levels [15]... 

Figure 17-5. Gradient separation of, in order of elntion, acetophenone, propiophenone, -bntyrophenone, valerophenone, hexanophenone, heptanophenone, and octanophe-none, performed on a Waters Acquity UPLC instrument. Column 2.1 x 100 mm, 1.7-pm ACQUITY BEH C18. Gradient 50-90% acetonitrile in 1.0 minutes. Column temperature 35°C.

Figure 8 UPLC-QTOF-MS base peak ion chromatogram obtained for the combined methanol extracts from soybean and Medicago truncatula (CV Jemalong A17). Separations were achieved using a Waters Acquity UPLC 2.1 x 100 mm, BEH C18 column with 1.7 pm particles, a flow of 600 jj.I min-1, and a linear gradient of 0.1% acetic acid acetonitrile (5 95 to 30 70 over 30 min). Mass spectra were collected on a Waters QTOFMS Premier. (Data generated by David Huhman.) Reprinted from M. Bedair L. W. Sumner, Trends Analyt. Chem. 2008, 27 (3), 238-250, Copyright (2008), with permission from Elsevier.

FIGURE 5.3 Comparison of XICs of acetaminophen-glucuronide (mlz = 328) (panels a and b) and acetaminophen-sulfate (ml7, = 232) (panels c and d) obtained using either a monolithic column (Panels a and c) or an ACQUITY UPLC column (panels b and d). Positive ion mode ESI-MS following injection of urine following administration of acetaminophen (APAP). (Reprinted from Johnson, K.A. and Plumb, R., J. Pharm. Biomed. Anal., 39, 805, 2005. With permission.)... 

Figure 1.2. (a) The traditional technique of low-pressure liquid chromatography using a glass column and gravity-fed solvent with manual fraction collection, (b) A modern automated HPLC instrument (Waters Acquity UPLC system) capable of very high efficiency and pressure up to 15,000 psi. 

PolyethCT Eggs and Amprolium C14H18N4 242.153146 243.160422 241.145870 260.186971 265.142367 Acquity UPLC Gradient profile mobile... 

Macro lides Honey Erythromycin A C37H67NO13 733.461244 734.468520 732.453968 751.495069 756.450465 Acquity UPLC Gradient profile mobile QqQ ESI+ [M- -H20+H]+ ... 

Acquity UPLC BEH C,8 column Acquity UPLC BEH Shield C18 (150 X 2.1 mm,... 

Acquity UPLC C,8 HSST3 (50x2.1 mm, 1.8 pm particle size)... 

Flow-rate 0.3 mL/min Column temperature 40°C Offline SPE Acquity UPLC BEH C,8 column... 

Acquity UPLC BEH C,5 column (50 X 2.1 mmi.d., 1.7 pm particle size) Gradient elution... 

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