Acidic domains

Isoform Mass (kDa) Gene name Amino acids Domains Domain location (approx.) Domain function Site of major expression Chromosome location... 

Boudin, H. and Craig, A. M. (2001) Molecular determinants for PICK1 synaptic aggregation and mGluR7a receptor coclustering role of the PDZ, coiled-coil, and acidic domains. J. Biol. Chem. 276, 30270-30276. 

Kawai, H., D. Wiedeeschain, and Z. M. Yuan, Critical contribution of the MDM2 acidic domain to p53 ubiquitination. Mol Cell Biol, 2003, 23(14), 4939-47. 

The production of chimeric cDNA molecules is typically used to localize an amino acid domain in a protein that gives rise to a specific property that is absent in a homologous protein. For example, this property could be the recognition of a specific drug, interaction with a specific protein, or localization to a specific cellular location. Chimeras are often a necessary first step in the path of identifying individual... 

Hart SL, Collins L, Gustafsson K, et al. Integrin-mediated transfection with peptides containing arginine-glycine-aspartic acid domains. Gene Ther 1997 4(11) 1225-1230. 

Enzyme amino acids domain sequence analysis performed... 

As has been clearly demonstrated, pi05 has two distinct ubiquitin system targeting motifs, an acidic domain that contains also the Lys residues essential for ubiquitin anchoring, and a C-terminal phosphorylation/E3-binding domain. An important, yet unresolved, question involves the biological rationale behind the evolution of these two sites. To resolve this... 

Along the mung bean hypocotyl, the cell wall plasticity represents the limiting factor of cell growth potentials. Pectin molecules, known to control local cell wall pH s and to modulate phenolic cross-linking owing to the number of free acidic domains, were investigated. 

There are several examples of metabolites where the tetramic acid domain is teasingly disguised. In these cases, it is difficult to be definitive about the nature of the apparent modification unless this is substantiated by biosynthetic studies. The case of lactacystin (5), Fig. (2), has already been considered. Another example is presented by the oxazolomycin group of antibiotics, e.g. (Ill), found in strains of Streptomyces [179]. Biosynthetic studies indicate that the carboxylic acid of the amino acid required to form tetramic acids contributes to the formation of the 3-lactone [180]. In this section, metabolites that probably have a tetramic acid origin are presented. 

In addition to structural domains devoted to DNA binding and dimerization (or oligomerization), many regulatory proteins must interact with RNA polymerase, with unrelated regulatory proteins, or with both. At least three different types of additional domains for protein-protein interaction have been characterized (primarily in eukaryotes) glutamine-rich, proline-rich, and acidic domains, the names reflecting the amino acid residues that are especially abundant. 

All of the oncosphere antigens cloned to date contain either one of two copies of a predicted Fnlll domain. These domains are widely distributed in eukaryotic proteins and occur also in some prokaryotic proteins (Bork and Doolittle, 1992). Approximately 2% of animal proteins include Fnlll domains. Many, but not all, of these proteins are extracellular and some have roles as adhesins. The structure of this 100 amino acid domain is highly conserved and consists of two layers with three p strands in one plane and four p strands in another (Potts and Campbell, 1996). Overall, amino acid sequence identity between different Fnlll domains is low, even between Fnlll repeat domains within fibronectin itself (Plaxco et al., 1997). Nevertheless, certain residues are highly conserved and maintain the tertiary structure of the proteins (Bork and Doolittle, 1992). Other conserved motifs such as an Arg-Gly-Asp (RGD) motif within a loop of some Fnlll domains is associated with proteins having cell adhesion properties, as discussed above (Ruoslahti and Pierschbacher, 1987 D Souza et al., 1991). 

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