Achieving DET of MCO by Rational Design

Conditions for achieving efficient DET via enzyme immobilization are dictated partly by materials architecture. Enzyme immobilization techniques may include nonspecific adsorption, covalent linkage, entrapment in conductive polymeric films, association with metal colloids, and encapsulation within porous matrices (see Chapter 11). The simplest method is nonspecific adsorption, but control is limited various noncovalent interactions will yield different orientations of the redox center with respect to the electrode interface and, as a result, inefficient DET. 

FIGURE 3.4 Dlustration of MCO immobilized onto PBSE-modified carbon nanotubes (a) and cyclic voltammograms (b) of laccase (i) and BOx (ii) modified electrodes. MCO physisorbed to carbon (1) and carbon/ MWCNT (2). MCO immobilized via PBSE to carbon/MWCNT with oxygen (3) and nitrogen (4). CV scans in phosphate buffer (pH 5.8), scan rate lOmV s . (Adapted with permission from Ref. [52]. Copyright 2010, Royal Society of Chemistry.) 

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