Acetobacter identification

The identification of terminal complexes in the Gram-negative bacterium Acetobacter xylinum now appears to be in doubt. Previously, a single linear row of particles observed on the outer lipopolysaccharide membrane... 

The sequences of citrate synthases from the eukaryotes pig heart and kidney, Arabidopsis thaliana and Saccharomyces cerevisiae, and from the eubacteria Escherichia coli, Rickettsia prowazekii, Acinetobacter anitratum, Acetobacter aceti and Pseudomonas aeruginosa have been determined (see the literature [85,88] for references). In addition, a high-resolution X-ray crystallographic structure is available for the pig heart enzyme [89,90]. This has allowed the identification of 12 residues which are critical for substrate binding and catalysis multiple sequence alignments [91] have indicated that the majority of these 12 active site residues are conserved between all eukaryotic and eubacterial citrate synthases. 

Trcek, J., Raspor, P., Teuber, M. 2000. Molecular identification of Acetobacter isolates from submerged vinegar production, sequence analysis of plasmid pJK2-l and application in the development of a cloning vector. Applied Microbiology and Biotechnology 53 289-295. 

Tanasupawat, S., Kommanee, J., Yukphan, R, Nakagawa, Y., Yamada, Y (2011). Identification of Acetobacter strains from Thai fermented rice productsbased onthe 16SrRNA gene sequence and 16S-23S rRNA gene internal transcribed spacer restriction analyses. Journal of the Science of Food and Agriculture, 91, 2652-2659. 

Li L, Wieme A, Spitaels L, Balzarini T, Nunes OC, Manaia CM, Van Landschoot A, De Vuyst L, Cleenwerck I, Vandamme P. Acetobacter sicerae sp. nov., isolated from cider and kefir, and identification of species of the gems Acetobacter by dnoK, groEL and rpoB sequence analysis. Int J Syst Evol Microbiol. 2014 64(Pt 7) 2407-15. doi 10.1099/ijs.0.058354-0. 

Saxena I.M. and Brown, Jr. R.M., 1995. Identification of a second cellulose synthase gene (acsAII) in Acetobacter xylinum. J Bacteriol 177 5276-5283. 

Lin F.C., Brown, Jr. R.M., Drake R.P., Jr., and Haley B.E. 1990. Identification of the uridine 5 -diphosphoglucose (UDP-glc) binding subunit of cellulose synthase in Acetobacter xylinus using the photoaffinity probe 5-azido-UDP-glc. J Biol Chem 265 4782- 784. 

Three bacteria were involved in the classical two-step fermentation process, for example G. oxydans, B. megaterium and K vulgare. The denomination of the bacteria for G. oxydans and K vuglare is highly speculative because of the lack of efficient strain identification methods. Furthermore, there are also some updates on the two strains. In early times, G. oxydans were named as G. melanogenus, Acetobacter suboxydans, or A melanogenus. Because most of these stains could not... 

Griffin AM, Morris VJ, J M (1996a) Identification, cloning and sequencing the aceA gene involved in acetan biosynthesis in Acetobacter xylinum. FEMS Microbiol Lett 137 115-121... 

Lisdiyanti P, Kawasaki H, Seki T, Yamada Y, Uchimura T, Komagata K (2(X)1) Identification of Acetobacter strains isolated from Indonesian sources, and proposals of Acetobacter syzygii sp. nov., Acetobacter cibinongensis sp. nov. and Acetobacter orientalis sp. nov. J Gen Appl Microbiol 47 119-131... 

Mehnaz S, Weselowski B, Lazarovits G (2006) Isolation and identification of Gluconacetobacter azotocaptans from comrhizosphere. Syst Appl Microbiol 29 496-501 Muthukumarasamy R, Revathi G, Seshadri S, Lakshminarasimhan C (2002) Gluconacetobacter diazotrophicus (syn. Acetobacter diazotrophicus), a promising diazotrophic endophyte in tropics. Curr Sci 83 137-145... 

Trcek J, Barja F (2015) Updates on quick identification of acetic acid bacteria with a focus on the 16S-23S rRNA gene internal transcribed spacer and the analysis of cell proteins by MALDI-TOF mass spectrometry. Int J Food Microbiol 196 137-144 Trcek J, Raspor P, Teuber M (2000) Molecular identification of Acetobacter isolates fiom submerged vinegar production, sequence analysis of plasmid pJK2-l and application in development of a cloning vector. Appl Microbiol Biotechnol 35 1899-1901 Trcek J, Toyama H, Czuba J, Misiewicz A, Matsushita K (2006) Correlation between acetic acid resistance and characteristics of PQQ-dependent ADH in acetic acid bacteria. Appl Microbiol Biotechnol 70(3) 366-373... 

Ameyama M, Kondo K (1958a) Carbohydrate metabolism by Acetobacter species. Part III. Isolation and identification of D-lyxuronic acid on glucose oxidation by A. melanogenum. Bull Agric Chem Soc Jpn 22(6) 380-386... 

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