Acetobacter suboxydans

Effect of Adenylic Acid and CO2 on Growth of Strept. hemolyticus C iOS S (277) Pressure of Oa 120mm. Hg. Total pressure made up to 740 mm. with Na. Growth given as (mg. bacterial N/10 ml. of culture) X 50. 

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A procedure has been reported473 for the direct determination, on a column of Polypak, of polyhydric compounds without conversion into derivatives. Separation of pentitols was not complete, but the method was found excellent for determining the amount of one alditol, as in the oxidation of L-arabinitol by Acetobacter suboxydans. This rapid method of monitoring the utilization of L-arabinitol permitted the reaction to be stopped as soon as all of the substrate had been consumed, before side reactions interfered. 

A recent important extension of the Hann, Tilden and Hudson rule has its origin in their observation that a reducing substance is produced by the action of Acetobacter suboxydans on L-fucitol this result was confirmed by Bollenback and Underkofler.167 Inspection of the stereoformula of L-fucitol (XCIII) shows a trans position for the hydroxyl... 

L-fucitol by it conforms with this extended interpretation of the Hann, Tilden and Hudson rule. Further studies of the action, or lack of action, of Acetobacter suboxydans on other w-deoxy-alditols are being reported169 and so far they indicate the general applicability of the rule under the extended interpretation that a terminal methyl group is to be regarded as qualitatively equivalent to a hydrogen atom, insofar as the specificity of the oxidation by Acetobacter suboxydans is concerned. 

The stereospecificity of the oxidation of cyclitols and deoxycyclitols by Acetobacter suboxydans has been defined by Magasanik, Franzl and... 

Alditols are oxidized by certain microorganisms for example, Acetobacter suboxydans converts D-mannitol into D-fructose the oxidation may occur... 

D-Glucose was reduced to the D-sorbitol with a hydrogen over Ni Raney, then it was turned into the L-sorbose with the acetobacter suboxydans and the hydroxyl groups of L-sorbose were protected with acetone treatment yielded the diaceton-L-sorbose. Subsequent treatment with NaOCI/Raney Ni produced di-O-isopropylidene-2-oxo-L-gulonic acid. Partial hydrolysis with aqueous HCI gave deprotected 2-oxo-L-gulonic acid, which yielded ascorbinic acid by heating with HCI. 

The Oxidation of Some Terminal-substituted Polyhydric Alcohols by Acetobacter suboxydans, L. Hough, J. K. N. Jones, and D. L. Mitchell, Can. ]. Chem., 37 (1959) 725-730. 

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