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A-tract DNA

A-tract DNA duplexes exhibit high propeller twist and rigidity, which makes A-tract DNA unfavourable for triplex formation. It has been demonstrated that the introduction of a single central GC pair into the duplex significantly enhances the stability of the resulting triplex, as does the introduction of dia-minopurine. In both cases, the stabilisation occurs due to the disruption of the intrinsic properties of A-tract DNA. [Pg.465]

Experiment to demonstrate that ax-[Pt(NH3)2 d(pGpG) ] intrastrand crosslinks bend duplex DNA by =34°. Panel A shows the platinated 22-mer sequence, panel B the effect of platination on the gel-electrophoresis mobility of the 22-mer (P22) and a control 27-mer (P27) oligomers, and panel C the mobility of copolymerized DNAs containing cis-DDP and A-tract induced bends ( , 128-bp Pt -t- A-tract DNA 0,96-bp Pt -P A-tract DNA) that maximize at approximately halfintegral helical turns corresponding to their phasing. For more detail consult Reference 93. [Pg.545]

The urocanamide derivative (96) has been assessed for stabilising triple-helices, and was shown to have specific recognition of a CG inversion of a homopurine-homopyrimidine duplex.The pteridone (97) has been used as a fluorescent sensor for melting transitions in A-tract DNA, where it was found to be more sensitive than in UV melting spectroscopy. ... [Pg.205]

Li, T, Jin, Y, Vershon, A. K. and Wolberger, C. (1998). Crystal structure of the MATal/MATa2 homeodomain heterodimer in complex with DNA containing an A-tract. Nucleic Acids Res. 26, 5707-5718. [Pg.240]

DNA Bending Assume that a polyf A) tract five base pairs long produces a 2CP bend in a DNA strand. Calculate... [Pg.303]

Most mature mRNAs have a post-transcriptionally added poly (A) tract at their 3 end (Wahle and Ruegsegger, 1999). Usually preceding the poly (A) addition site is a sequence transcribed from the sequence AATAAA on the DNA. This sequence, the so-called poly (A) signal, is the most conserved recognition element in eukaryotes. This sequence motif directs the cleavage of primary transcripts... [Pg.5]

DNA Bending Assume that a poly(A) tract five base pairs long produces a 20° bend in a DNA strand. Calculate the total (net) bend produced in a DNA if the center base pairs (the third of five) of two successive (dA)5 tracts are located (a) 10 base pairs apart (b) 15 base pairs apart. Assume 10 base pairs per turn in the DNA double helix. [Pg.90]

Mollegaard NE, Bailly C, Waring MJ, Nielsen PE. Effects of diaminopurine and inosine substitutions on A-tract induced DNA curvature. Importance of the 3 -A-tract junction. [Pg.245]

Three-center (bifurcated) hydrogen bonding between adjacent base pairs explains unusual properties of poly(dA) poly(dT). The stability of the B-form of this duplex (and of its ribo analog) formed by two homopolymers might be a consequence of the extreme propeller twist (Box 20.1) reported for the central portions (the A-tracts) of the double helical dodecamer d(CGCAAAAAAGCG) [702] and d(CGCAAATTTGCG) [703]. In both crystal structures the DNA molecules show the same unusual three-center bonds linking adjacent base pairs. [Pg.410]

Based on the geometry of the central A-tract, a double helix was constructed. Compared with normal B-DNA, it displays a very narrow, deep minor groove and a wide, shallow major groove and has a pitch of 34 A with 10.0 base pairs per turn. The three-center hydrogen bonding which links all the base pairs of the double helix in axial direction confers extra stabilization to this double helical structure, which explains several experimental findings not understood previously [702, 703]. [Pg.410]

A few deaza- and aza-dA analogues are reported. 3 -Deaza-dA has been used to probe minor groove recognition contacts,as well as its interactions in DNA curvature at A-tracts. 1-Deaza-adenosine, tubercidin, purine and 4-methylindole deoxyribosides have all been used to probe for an essential... [Pg.724]

See other pages where A-tract DNA is mentioned: [Pg.514]    [Pg.671]    [Pg.3]    [Pg.150]    [Pg.150]    [Pg.151]    [Pg.152]    [Pg.154]    [Pg.154]    [Pg.154]    [Pg.155]    [Pg.155]    [Pg.159]    [Pg.164]    [Pg.221]    [Pg.514]    [Pg.671]    [Pg.3]    [Pg.150]    [Pg.150]    [Pg.151]    [Pg.152]    [Pg.154]    [Pg.154]    [Pg.154]    [Pg.155]    [Pg.155]    [Pg.159]    [Pg.164]    [Pg.221]    [Pg.408]    [Pg.52]    [Pg.97]    [Pg.97]    [Pg.100]    [Pg.248]    [Pg.98]    [Pg.55]    [Pg.82]    [Pg.366]    [Pg.134]    [Pg.462]    [Pg.146]    [Pg.138]    [Pg.90]    [Pg.347]    [Pg.268]    [Pg.283]    [Pg.495]    [Pg.499]    [Pg.742]   


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A-DNA

A-tract

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