A-tracts

A) tracts, whereas conformation of the probe changed in the presence of an RNA poly(A)tract (alteration on the micropolarity around a pyrenecarboxamide chromo-phore caused by hybridization with the targeted RNA) and a much higher fluorescence was observed. 

Fluorescence measurement using this probe does not require a fluorescence quencher or washing process to suppress the fluorescence emission from nonbinding probes and nonspecific binding probes, which would be advantageous for the detection of mRNAs with poly(A) tracts in cells. 

Tanaka K, Okamoto A (2008) Design of a pyrene-containing fluorescence probe for labelling of RNA poly(A) tracts. Bioorg Med Chem 16 400-404... 

Translational efficiency of luciferase mRNAs lacking a poly(A) tract and capped with anti reverse cap analogs in a RRL system. The relative translational efficiency was calculated as described in the text. Translational efficiency of luciferase mRNAs containing 60-nt poly (A) tract and capped with anti reverse cap analogs in MM3MG cells. Luciferase activity was normalized by the amount of luciferase RNA in the cells. Relative translational efficiency was calculated as noted previously. 

Degradation of 5 -terminal sequences in luciferase mRNA containing 60-nt poly(A) tract and capped with the indicated anti reverse cap analogs. The mRNAs half-lives were determined by real time PCR with primers directed against the 5 -end of luciferase mRNA as described in the text. 

The nature of the cap structure can also influence mRNA stability. The stability of mRNA capped with different ARCAs is determined in MM3MG cells that are electroporated with luciferase mRNAs containing a 60-nt poly(A) tract and different cap analogs. The experiment is done... 

Frederick had become so deeply infected by irreligion that he d written a tract called Virtue, Immortality of the Soul, and the Reliability of the Bible, which he d later destroyed to keep from her eyes. There seemed no doubt that Fritz had been questioning the very existence of God. Elisa trembled at the dangers of such profanity. The soul of her brother was in mortal peril. 

Reynolds, P., Weber, S., and Prakash, L. RAD6 gene of Saccharomyces cerevisiae encodes a protein containing a tract of 13... 

Li, T, Jin, Y, Vershon, A. K. and Wolberger, C. (1998). Crystal structure of the MATal/MATa2 homeodomain heterodimer in complex with DNA containing an A-tract. Nucleic Acids Res. 26, 5707-5718. 

DNA Bending Assume that a polyf A) tract five base pairs long produces a 2CP bend in a DNA strand. Calculate... 

Most mature mRNAs have a post-transcriptionally added poly (A) tract at their 3 end (Wahle and Ruegsegger, 1999). Usually preceding the poly (A) addition site is a sequence transcribed from the sequence AATAAA on the DNA. This sequence, the so-called poly (A) signal, is the most conserved recognition element in eukaryotes. This sequence motif directs the cleavage of primary transcripts... 

Niitzel was instrumental in the move to take control of monastic orders and their properties. In 1524 he was made guardian of St. Clare and published a tract outlining plans for... 

The ribosome has a problem similar to that of RNA polymerase the ribosome must find the point in the mRNA at which to begin translation. In prokaryotes the site is marked by a tract called the Shine-Dalgarno sequence, about ten nucleotides upstream from the initiation site. Initiation occurs at the first subsequent AUG sequence. (AUG codes for the amino acid methionine.) In eukaryotes, initiation usually begins simply at the first AUG sequence from the 5 -end of the mRNA. 

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