35S labelling

Cottrill, S.M., Sharma, H.L, Dyson, D.B., Parish, R.V. and McAuliffe, C.A. (1989) The role of the ligand in chrysotherapy a kinetic study of gold-199- and sulfur-35-labeled myocrisin and auranofin. Journal of the Chemical Society, Perkin Transactions II, (1), 53-58. 

Gunina AI. 1957b. [Transformation of sulfiir-35-labeled hydrogen sulfide introduced into blood.] Dokl AkadNauk SSSR 112 902-904. (Russian)... 

Marcotrigiano, G. Peyronel, G. Battistuzzi, R. 1972. Kinetics of desulfuration of S-35-labeled thiourea in sodium hydroxide studied by chromatographic methods. /. Chem. Soc., Perkin Trans. 2 1539-1541. 

Fig. 1. Transmission mechanisms. Strain barrier PrPc (circle) interacts with different strains of PrPSc (square or triangle). The replicated PrPSc is similar to the template. The 3F4 epitope is not recognized when it is in PrPSc, but is exposed after pardal denaturation by GdnHCI so that it is detected by the antibody. Antibody reactivity depends on the particular strain of PrP (Safar et aL, 1998). Species barrier when the template PrPSc contains unfavorable residues at the binding interface, the transformation of PrPc to Pr l>Sr does not occur. In vitro replication 35S label of PrPc is detected in PrPSc after replication in a medium containing GdnHCI (Kocisko et aL, 1994).

Inhibitors known to be selective for MAO A or B have been labeled with P F]-fluorine for PET studies. For example, [ F]-fluoroclorgyline (35) labeled on the aromatic ring with low specific activity [ F]-fluorine was shown to have favorable properties for mapping MAO A activity in the brain [103],... 

Figure 5-49 A DNA sequencing gel obtained using a segment of DNA from salmon sperm selected by suitable oligonucleotide primers, amplified by PCR, and sequenced with a 35S label in the primer. Four samples were used, one with each of the four dideoxy chain terminators (A, G, C, T, A, C, G, T from left to right). After electrophoresis the shorter fragments are at the lower end of the gel. The sequence of the strand complementary to the template strand whose sequence is being determined is read from the bottom of the gel. Here it starts CTATGATAC. Reproduced by permission of Amersham Pharmacia Biotech, Limited.

Dixon et al. measured the emission into air, above an aqueous solution of the surface active compound, of soft / -rays of a 35S labelled soap. 

Academic Press (Orlando, FL), pp. 25-29. Safety with 32P- and 35S-Labeled... 

R Zoon, in Methods in Enzymology, Vol. 152, S. Berger and A Kimmel, Editors (1987), Academic Press (Orlando, FL), pp. 25-29 Safety with 32P- and 35S-Labeled Compounds ... 

Experiments with 35S-labelled sulfate solutions have shown that part of the extracted uranium is transferred across the interface as the neutral species U02S04, especially at low concentrations of aqueous sulfate 203 the principal extraction reactions for the secondary amine used, di- -decylamine, are reported to be... 

Sulphur-35 resembles carbon-14 in many respects. It is a low-energy -emitter with half-life of 87 days. It is available as S04 at high specific activity and as elementary sulphur at lower activity. Many 35S-labelled compounds can be obtained commercially. 

Thode-Andersen, S. and Jprgensen, B.B. (1989) Sulphate reduction and the formation of 35S-labeled FeS, FeS2, and S° in coastal marine sediments. Limnol. Oceanogr. 34, 793-806. 

J.L. Hambrook, J.M. Harrison, D.J. Howells and C. Schock, Biological fate of sulfur mustard (l,l -thiobis(2-chloroethane)) urinary and faecal excretion of 35S by rat after injection or cutaneous application of 35S-labelled sulfur mustard, Xenobiotica, 22, 65-75 (1992). 

Fio. 2. Plasmin and uPA-catalyzed cleavage of suPAR(I-III). (A) 35S-labeled recombinant suPAR(I-III) was purified on an immunoaffinity column with the domain I-specific mAb R3 immobilized [86], Lane 1 is 10 nM suPAR(I-III), lane 2 is 10 nM suPAR(I-III) incubated for 2 hours at 37 °C with 50 nM plasmin (pli), lane 3 is 10-nM suPAR(I-III) incubated for 20 hours at 37°C with 500-nM uPA, and lane 4 is 35S-labeled domain I purified from cell culture media on an immunoaffinity column with the domain I-specific mAb R9 immobilized, after removing suPAR (I—III) from the media by immunoaffinity chromatography, employing the domain Ill-specific mAb R2. (B) Ten nM suPAR(I-III) was preincubated with 67 nM of the indicated mAbs for 2 hours at 37°C prior to addition of either 50 nM plasmin (lanes 2,3,4, and 6) or 500 nM uPA (lane 5) and continued incubation for 2 or 20 hours at 37 °C. Lane 1 is suPAR(I-III) alone, lane 2 is suPAR(I-III) incubated with plasmin only, lane 3 shows preincubation with a subtype control mAb, lanes 4 and 5 show preincubation with mAb R3, lane 6 shows preincubation with the domain Ill-specific mAb R4. The radioactive samples have been separated by SDS-PAGE prior to autoradiography of the dried gel. 

Figure 7.11. The effects of exposure temperature on protein synthetic patterns of isolated gill tissue from specimens of 13°C-acclimated Tegula funebralis. Autoradiographic images illustrate newly synthesized (35S-labeled) proteins of several size classes (molecular mass standards are shown in the left lane). Two specimens from each temperature of incubation are shown. At temperatures above 24°C, synthesis of heat-shock proteins in the molecular mass ranges of 38, 70, 77, and 90 kDa is induced. Hsp synthesis becomes an increasingly large fraction of protein synthesis as exposure temperature increases, and by 38°C, only synthesis of hsp70 is observed. By 39° C, no protein synthesis takes place. (Figure modified after Tomanek and Somero, 1999.)...

Work with labeled surfactants at Davis, Fort Detrick, and VPI by both authors has shown that with nonionic surfactants such as Tween 20 and Tween 80 very few of the tagged atoms are translocated away from the site of application (Figure 4). For Tween 20 less than 2-3% were found outside the treated leaf after 7 days (Table II). Even in cases where translocation of label had occurred such as with 35S-labeled sodium lauryl sulfate the label in remote parts of the plant was not in the form of the original surfactant molecule but was in other metabolites. 

Ullberg S (1954) Studies on the distribution and fate of S-35-labelled benzylpenicillin in the body. Acta Radiologica 1954 (suppl)118 l-110... 

Protein, Nucleic Acid And Enzyme 39(11) 181—191 Steinke W, Archimbaud Y, Becka M, Binder R, Busch U, Dupont P, Maas J (2000) Quantitative Distribution Studies in Animals Cross-Validation of Radioluminography versus Liquid-Scintillation Measurement. Regulatory Toxicology and Pharmacology 31(2) 33-43 Ullberg S (1954) Studies on the distribution and fate of S-35-labelled benzylpenicillin in the body. Acta Radiologica 1954 (suppl)l 18 1—110... 

To circumvent this problem it was found advantageous to use a slight excess of sodium hydroxide in the reaction, i.e., a 5% excess of the required 6 molar equivalents. This ensures that the solution is basic enough to prevent decomposition of the sodium phosphorothioate once it is formed. Yasuda and Lambert reported a percentage yield of 57% of the theoretical. In a series of three preparations on roughly a 1-mol scale where 5% excess base was used, we obtained an average yield of 67%. It was also possible to scale down the reaction to permit synthesis of 35S-labeled sodium phosphorothioate. In a series of four syntheses on a 4-5-millimol scale an average yield of 59% was achieved. 

Label each of the species in the following equations as an acid or a base, and connect the conjugate pairs ... 

Clemedson, C.J., Kristofferson, H., Sorbo, B. et al. (1963). Whole body autoradiographic studies of the distribution of sulphur 35-labelled mustard gas in mice. Acta Radiol. Ther. Phys. Biol. 1 314-20. 

Deoxyribonucleotides (dATP, dTTP, dGTP, dCTP). Deoxyribonucleotides are the substrates of all DNA polymerases. The dideoxy method utilizes reactions, where the enz3une catalyzes polymerization of these nucleotides into new strands of DNA. The dATP that is used actually is not dATP, but a similar compotmd that is radioactive. This compound is sulfur-35-labeled dATP. Only the dATP is spiked with The other deoxyribonucleotides are not radioactive. 

The application of the solvent system concept to liquid sulfur dioxide chemistry stimulated the elucidation of reactions such as those of aluminum sulfite. However, there is no direct evidence at all for the formation of SO in solutions of thionyl halides. In fact, there is evidence to the contrary. When solutions of thionyl bromide or thionyl chloride are prepared in 35S-labeled (S ) sulfur dioxide, almost no exchange takes place. The half-life for the exchange is about two years or more. If ionization took place ... 

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