Yeasts disinfectant tests

The efficacy tests used are the MIC test and other tests based on the same principle as the disinfectant tests (see Sec. II. B). The bacteria used in the tests are chosen among strains representative of those found in the home for example, bacteria from the Pseudomonas family, the Enterobacteriaceae, yeast such as Candida, molds such as Aspergillus niger, and others. 

A standardized viral suspension is exposed, in the presence of yeast suspension, to appropriate dilutions of disinfectant in WHO hard water. At appropriate times, dilutions are made in inactivated horse serum and each dilution is inoculated into tissue cell culture or embryonated eggs (as appropriate for the test virus). The drop in infectivity of the treated virus is compared with that of the control (untreated) virus. 

Level 1 concerns basic suspension tests, performed with disinfectant dilutions to show if the product is disinfectant or not, and at what concentration. The target is to achieve a 5 log reduction for bacteria and a 4 log reduction for yeast and molds within 1 h (see Table 1). 

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