Yeast plasma membranes

FIGURE 10.18 A model for the structure of the a-factor transport protein in the yeast plasma membrane. Gene duplication has yielded a protein with two identical halves, each half containing six transmembrane helical segments and an ATP-binding site. Like the yeast a-factor transporter, the multidrug transporter is postulated to have 12 transmembrane helices and 2 ATP-binding sites. 

Arndt and Ahlers [38] used X-ray powder diffraction method for studying the influence of cations on the mode of action of miconazole on yeast cells. The influence of miconazole nitrate on yeast plasma membranes was studied in a concentration range of 0-100 pM. The reaction of 100 pM miconazole with the... 

Figure 7.5 Summary of iron uptake across the yeast plasma membrane. (From Kosman, 2003. Reproduced with permission of Blackwell Publishing Ltd.)...

Kwok, E.Y., Severance, S. and Kosman, D.J. (2006) Evidence for iron channeling in the Fet3p-Ftrlp high-affinity iron uptake complex in the yeast plasma membrane, Biochemistry, 45, 6317-6327. 

Hicke, L. and Riezman, H. Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis. Cdl 1996, 84, 277-87. 

R. Deubiquitination step in the endocytic pathway of yeast plasma membrane proteins crucial role of Doa4p ubiquitin isopeptidase, Mol Cell Biol, 2001, 21, 4482-94. 

Lecchi, S. Allen, K. E. Pardo, J. P Mason, A. B. Slayman, C. W. Conformational Changes of Yeast Plasma Membrane H+-ATPase during Activation by Glucose Role of Threonine-912 in the Carboxy-Terminal Tail. Biochemistry 2003, 44, 16624-16632. 

Galan, J., and R. Haguenauer-Tsapis. Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein. Embo J. 16 5847-54.1997. 

Galan JM, Cantegrit B, Garnier C, Namy O, Haguenauer-Tsapis R (1998) ER degradation of a mutant yeast plasma membrane protein by the ubiquitin-proteasome pathway. FASEB J 12 315-323... 

Hicke L (1999) Gettin down with ubiquitin turning off cell-surface receptors, transporters and channels. Trends Cell Biol 9 107-12 Hicke L, Riezman H (1996) Ubiquitination of a yeast plasma membrane receptor signals its ligand-stimulated endocytosis. Cell 84 277-287 Hicke L, Zanolari B, Riezman H (1998) Cytoplasmic tail phosphorylation of the a-factor receptor is required for its ubiquitination and internalization. J Cell Biol 141 349-358... 

Portillo, F. Serrano, R. (1989). Growth control strength and active site of yeast plasma membrane ATPase studied by site-directed mutagenesis. Eur. J. Biochem. 186, 501-507. 

FetSp and Ftrlp most hkely form a heterodimeric complex in the yeast plasma membrane (Stearman et al., 1996). FetSp and Fthlp form a homologous complex in the vacuolar membrane (Urbanowski and Piper, 1999). The evidence for this model is indirect in that a specific physical interaction between the two proteins has not been directly demonstrated. The lack of evidence for a similar functional interaction between Cp and Tf was noted above. FetSp and Ftrlp do localize to the plasma membrane. However, the most compelling evidence for a physical interaction between the two is that this localization depends on the presence of both proteins if one is not produced, then the other remains intracellular (Stearman et al., 1996). Furthermore, in the absence of Ftrlp production, the FetS protein that is made remains in an enzymatically inactive form, presumably as the a/io-protein. 

Model of Fet3p-Ftr1 p Complex Yeast Plasma Membrane Type 1 Cu(ll) FE(III) - Transferred to... 

Fig. 16. Models for the membrane topology and orientation for the FetSp/Ftrlp and Fet5p/Fthlp complexes in yeast membranes. (Top) FetSp/Ftrlp are pictured in the yeast plasma membrane oriented to the extracellular space. The (R)EXXE motifs that may be involved in iron uptake and the transmembrane domain required for correct assembly are indicated. These roles have been suggested by mutagenesis studies (Stearman et al., 1996). (Bottom) Model of Fet5p/Fthlp complex proposed for the membrane of the yeast vacuole oriented to the lumen (inside) of the vacuole. In this model, the EXXE motifs in Fthlp are not oriented toward the ferroxidase site on Fet5p (Urbanowski and Piper, 1999).

Jones, R.P., Greenfield, PE, (1987) Ethanol and the fluidity of the yeast plasma membrane. Yeast, 3, 223-232. 

R. Serrano, M.C. Kielland-Brandt, G.R. Fink, Yeast Plasma Membrane ATPase Is Essential for Growth and Has Homology with (Na+ + K+), K and Ca -ATPase , Nature, 319, 689 (1986)... 

Regulation of turgor pressure In plant tonoplast Plant plasma membrane Yeast plasma membrane... 

Desmosmal and Intermediate junction proteins Erythrocyte membrane and membrane-skeleton Fungal (yeast) plasma membrane Gap junction channel proteins (connexon)... 

In addition to its well established role in targeting proteins for degradation, ubiquitination has also been shown to play a role in cellular signalling. Thus, ubiquitination of the yeast plasma membrane receptor, Ste2p (a G-protein-coupled receptor that binds a mating pheromone), acts as a signal for its ligand-stimulated endocytosis. 

Serrano R. Kielland-Brandt MC, Fink CR. Yeast plasma membrane ATPase is essential for growth and has homology with (Na + K ), K - andCa -Atpases. Nature 1986 319 689-693. 

S. Lecchi, E.A. Kenneth, J.P. Pardo, A.B. Mason, C.W Slayman, Conformational changes of yeast plasma membrane H -ATPase during activation by glucose Role of threonine-912 in the carboxy-terminal tail, Biochemistry 44 (50) 16624-16632,2005. 

Contribution of 0 Technology to the Mechanism of the H -ATPase from Yeast Plasma Membrane Antoine Amory, Andr6 Goffeau,... 

Holoubek, A. Vecer, J. Opekarova, M. Sigler, K. Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H -ATPase reconstituted into vesicles. Biochim. Biophys. Acta, Biomembr. 2003, 1609, 71-79. 

Dufour, J. R Goffeau, A. Tsong, T. Y. Active proton uptake in lipid vesicles reconstituted with the purified yeast plasma membrane ATPase. Eluorescence quenching of 9-amino-6-chloro-2-methoxyacridine. J. Biol. Chem. 1982, 257, 9365-9371. 

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