Wilkins, Marc

Kreek, Jeanne, and Marc Reisinger. 1997. "The Addict as Patient." In Substance Abuse A Comprehensive Textbook, edited by Joyce H. Lowinson, Pedro Ruiz, Robert B. Millman, and JohnG. Langrod. Baltimore, Md. Williams and Wilkins. Kuehule, John, and Robert Spitzer. 1992. "DSM III Classification of Substance Use Disorders." In Substance Abuse A Comprehensive Textbook, edited by Joyce... 

Ten years ago Marc Wilkins, at that time an Australian student, coined the word proteome. Since then this research field has evolved ever more rapidly and continues to do so. Worldwide, many conferences and research programmes cover this topic and a series of new journals are publishing more and more proteomics articles. 

The term proteome was first used by Marc Wilkins in 1994 (Wilkins 1996). An effort to describe the total proteins of an organism was made independently by O Farrell (1975) and by Klose (1975). They developed what is called two-dimensional (2D) gel electrophoresis by running gel electrophoresis of proteins in two planes at right angles to each other (O Farrell 1975, Klose 1975). This method separated a complex mixture of more than 1100 proteins of Escherichia coli into distinct bands of individual components on the gel. Later, the science of proteomics was revolutionized by the application of mass spectrometry in conjunction with genomics for the separation and identification of proteins on a large scale. 

In 1994, Marc Wilkins threw the term proteome into the scientific community. And the word became flesh. Today, proteome and proteomics are as hip as PCR was at the end of the 1980s. The word proteome is defined as the quantitative totality of proteins of a cell, tissue, or organism (i.e., the knowledge of all expressed proteins and their respective concentrations under certain external conditions). 

Swanson, Todd A., Sandra I. Kim, and Marc J. Glucksman. Biochemistry and Molecular Biology, 4th ed. Lippincott Williams Wilkins, Baltimore, 2007. 

The term proteome was first coined in late 1994 by Marc Wilkins at the Siena two-dimensional gel electrophoresis (2DE) meeting and defines the entire protein complement in a given cell, tissue, or organism [1], In its wider sense, proteomics research also assesses protein activities, modifications and localization, and interactions of proteins in complexes. It relies heavily on technology since it needs to identify proteins and protein complexes in biological samples comprehensively and quantitatively with both high sensitivity and fidelity. 

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