Whole genome shotgun

Mural, R.J., Adams, M.D., Myers, E.W., et ah A comparison of whole-genome shotgun-derived mouse chromosome 16 and the human genome. Science 296, 1661— 1671, 2002. 

G. Elgar, T. Hawkins, B. Venkatesh, D. Rokhsar and S. Brenner. Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science 297 1301 -1310, 2002. 

In the second strategy called whole-genome shotgun sequencing (WGS), the shotgun sequencing method is applied to the whole genome. Multiple... 

Istrail, S., G. G. Sutton, L. Florea, A. L. Halpern, C. M. Mobarry, R. Lippert, B. Walenz, et al. 2004. Whole-genome shotgun assembly and comparison of human genome assemblies. Proc Natl Acad Sci USA 101 1916-21. 

The international public (HGP) and American private (Celera) efforts to determine the sequence of the human genome exploited different approaches [9,10,35], It is still not clear whether Celera s whole genome shotgun sequencing (WGC) approach was really efhcient as they used publicly available data and it is not known to what extent. 

Figure 1.2-7. Two main approaches used to sequence the human genome. The hierarchical approach is based on creation of clone contigs covering the whole genome and shotgun sequencing of individual cosmid/BAC/PAC clones (a). The whole genome shotgun sequencing scheme (WGS) shown in (b) is based on pair-end sequencing of small-insert and selected BAC/PAC clones. See text for details.

First proposed by James Weber and Eugene Myers before Celera existed and later eagerly adopted by the company. See Weber, J.L., Myers, E.W. Human whole-genome shotgun sequencing. Genome Res. 1997, 7, 401 09. 

J, Bohlmann J, Jones SJM (2013) Assembling the 20 Gb white spruce (Picea glauca) genome from whole-genome shotgun sequencing data. Bioinformatics 29 1492-1497... 

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