Water-soluble vitamin HPLC chromatogram

FIGURE 16 HPLC chromatogram of water-soluble vitamins using ion-pair chromatography. LC conditions and peak identification are shown in the inset.The retention times of basic analytes (pyridoxine and thiamine) are strongly dependent of the concentration of ion-pairing reagent (1-hexanesulfonate) in the mobile phase. Reprinted with permission from Reference 17. 

FIGURE 7 HPLC chromatogram of water-soluble vitamin in multivitamin tablets. See Reference 33 for details. Reprinted with permission from Reference 33. 

Figure 6.5. HPLC chromatograms showing the analysis of water-soluble vitamins in multivitamin capsules tablets. Reprinted with permission from reference 17.

An HPLC particle beam PCI-MS chromatogram of a water-soluble vitamin standard mixture is shown in Figure 29. 

In this procedure, the HPLC analysis is carried out on a DuPont Zorbax ODS column using methanol, acetonitrile and water 10 2 1 at the mobile phase. This system resolves vitamins D3 from D2 and from degradation products and other fat soluble vitamins. Didecyl and dinonyl phthalate were used as the internal standards. The latter was included for use in the event that extraneous peaks interfered with the didecyl phthalate peak. Figure 11 shows a chromatogram of an extract of a multivitamin formulation with the two added internal standards. 

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