VP-hPXR

Ugtlal f (L WT, VP-hPXR, hPXR mice) Ugtla9 f (L WT mice)... 

FIGURE 7.4 Creation of VP-PXR transgenic mice. Schematic representation of the Alb-VP-hPXR transgene construct, and transgene expression in the liver of transgenic mice. 

Alb-VP-hPXR Transgenic Mice Expressing the Activated hPXR... 

FABP-VP-hPXR transgenic mice were created to express the constitutively activated hPXR (VP-hPXR) in both the liver and intestine. Figure 1.5a shows the schematic representation of the transgene. Transgene expression was evaluated by Northern blot analysis using an hPXR-specific probe. As shown in Figure 1.5b, VP-hPXR was... 

FIGURE 7.5 Creation of FABP-VP-hPXR transgenic mice, (a) Schematic representation of the transgene construct. (b) Northern blot analysis shows that the VP-hPXR transgene is expressed in the liver and throughout the intestinal tract. The membrane was probed with hPXR cDNA, mPXR, and GAPDH for the loading control. 

The FABP-VP-hPXR mice were subsequently used to confirm that, like their Alb-VP-hPXR counterparts, activation of hPXR in this transgenic line also resulted in the disruption of adrenal steroid homeostasis [40] and promotion of hepatic steatosis [31]. 

---