Vitamin determination with antimony

The standard chemical and biological methods of analysis are those accepted by the United States Pharmacopeia XXlll as well as the ones accepted by the AO AC in 1995 (81—84). The USP method involves saponification of the sample (dry concentrate, premix, powder, capsule, tablet, or aqueous suspension) with aqueous alcohoHc KOH solvent extraction solvent removal chromatographic separation of vitamin D from extraneous ingredients and colormetric determination with antimony trichloride and comparison with a solution of USP cholecalciferol reference standard. 

Direct UV-visible spectrophotometric determination of vitamin D is limited to pure dosage forms that should not even contain other fat-soluble vitamins. In an older colorimetric AOAC procedure the absorbance of a colored product resulting from the reaction of vitamin D with antimony trichloride was measured at 500 nm. However, the other fat-soluble vitamins, particularly vitamin A, as well as the... 

Dwivedi and Arnold (89) determined the thiazole moiety in thiamine by derivatizing first to form the TMS analog. Samples were chromatographed on 3% OV-17 on Chromosorb G (DMCS treated) at 110°C. Vitamin D2 (calciferol) and its analog Vitamin have been the subject of many applications in GC analyses. The compounds have been derivatized as the TMS analog and chromatographed on OV-17 (90) and SE-30 (91) and on 3% silicone on Celite after treatment with antimony trichloride (92). Several references to vitamine E (oi-tocopherol) are included in the review by Kern et al. (39) on GLC determinations of pharmaceuticals and drugs. The acetate was determined on 3% OV-17 on Chrom W-HP at 280°C. Vecci and Kaiser (93) determined Vitamin C as the TMS derivative on 3% SE-30 or 10% XE-60 on Anachrom ABS. Column... 

Many nutrients are analyzed by chemical procedures which involve either colorimetry or spectrophotometry. Vitamin A analysis is a good example of a colorimetric procedure. The standard assay for vitamin A determination involves the treatment of the sample with antimony trichloride. A deep blue-colored solution is produced, the intensity of which is... 

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