Vectors lacZ gene

Ligated plasmids should be checked for insertion of the desired fragment instead of just religation, most conveniently by blue-white screening. In vectors with the lacZ gene sequence, insertion at any restriction site of the MCS results in disruption of that sequence so that no /i-galactosidase is expressed and the synthetic X-gal substrate cannot form blue colonies. 

The pUC vectors contain an ampicillin-resistance gene and a multiple cloning site at the 5 -end of the lacZ gene. Insertional inactivation of lacZ allows... 

TABLE 8.2 Some Commercial Plasmid Vectors Expressing Full-Length lacZ Gene... 

Andersen JK, Garber DA, Meaney CA, Breakefield XO (1992) Gene transfer into mammalian central nervous system using herpes virus vectors Extended expression of bacterial lacZ in neurons using the neuron-specific enolase promoter. Hum Gene Ther 3 487-499. 

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