Multiple cloning site

Molecular Biology for Improved Biocatalysts 99 Promoter Multiple cloning site... 

Fig. 1. The expression vector pTDI. (a) pTDI vector map. (b) DMA sequence of the pTDI vector around the multiple cloning sites.

Expression vector with suitable promotor, multiple cloning site, and fusion tag, where applicable (e.g., six-histidine tag). 

Moment and disposition parameter, 421 Multiple cloning sites (MCS), 9 Multiple RNA export pathways, 267 Multi-valent cation, 140... 

The E. coli expression system used is based on the pQE-80L expression system. Sequences inserted into the multiple cloning site can be expressed as native proteins bearing an N-terminal hexahistidine tag upon IPTG induction of the T5 promoter in suitably transformed E. coli cells. 

HEK-293 hGH High-Five hsp70 HSV IPTG IRES kb Lac LCR LoxP LUC MCS human embryonic kidney cells human growth hormone TM BTI-TN-5B1-4 (cell line derived from the insect Trichoplusia ni) heat shock protein 70 herpes simplex virus isopropyl 1 -thio-fi-D-galactopyranoside internal ribosomal entry site kilobases lactose operon/repressor locus control region locus of crossover of PI luciferase isolated from firefly multiple cloning site... 

Targeting vector A vector containing a positive selection marker (e.g., neomycin-resistance gene), negative selection marker (thymidine kinase gene) and a multiple cloning site. 

Over several decades, multiple vector systems for recombinant gene expression in E. coli have been developed. Modem vectors suitable for recombinant protein production vary in the used promoter system in the presence or absence of coding sequences for affinity tags upstream or downstream of the multiple cloning site (MCS) and of sequences coding for leader peptides for the protein export. Moreover, different origins of replication (ori), antibiotic selection marker genes and MCS are used. 

Fig. 4 Plasmids for the intracellular production of tagged proteins in B. megaterium. All plasmids of the series 1622 are based on the shuttle vector pSTOP1522 (Fig. 3). (a) DNA sequence of ribosome binding site (RBS) and multiple cloning site (MCS) of the expression plasmid pSTOP1622. The...

Figure 10-2 The pGEX-2T plasmid. Multiple-cloning site allows genes of interest to be inserted in frame with the GST coding sequence to express the gene as a GST fusion protein that is easily purified with affinity chromatography. The GST tag can be removed after purification by treatment with thrombin.

Notice that the pGEX-2T plasmid contains a multiple cloning site immediately 3 to the GST coding sequence. If a gene of interest is cloned into... 

Multiple cloning site contains a number of unique, closely spaced restriction endonuclease recognition sites where foreign DNA of interest may be inserted... 

---