Vector for DNA

See also Embryo cloning Cloning vectors, for DNA fragments, 22 504-506... 

Sequential hydroformylation/reductive amination of dendritic perallylated polyglycerols with various amines in a one-pot procedure to give dendritic polyamines in high yields (73-99%). Furthermore, the use of protected amines provides reactive core-shell-type architectures after deprotection. These soluble but membrane filterable multifunctional dendritic polyamines are of high interest as reagents in synthesis or as supports in homogeneous catalysis as well as nonviral vectors for DNA-transfection (Scheme 18) [65]. 

Fischer, D., Bieber, T., Li, Y., Elsasser, H-P, Kissel, T. (1999). A novel non-viral vector for DNA delivery based on low molecular weight, branched polyethylenimine effect of molecular weight on transfection efficiency and cytotoxicity. Pharm. Res., 16, 1273-12791. 

What feature of circular, double-helical DNA is essential for it to function as a vector for DNA cloning ... 

Plasmids and Lambda Phage Are Choice Vectors for DNA Cloning in Bacteria... 

Kunath K, et al. (2003). Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery Comparison of physicochemical properties, transfection efflciency and in vivo distribution with high-molecular-weight polyethylenimine. J. Control. Rel. 89 113-125. 

Lo SL, Wang S (2008) An endosomol54ic Tat peptide produced by incorporation of histidine and cysteine residues as a nonviral vector for DNA transfection. Biomaterials 29 2408-2414... 

Plasmid-. Small circular DNA molecule that can replicate independently of the genomic DNA. Used extensively as a vector for DNA cloning. Is usually amplified in E. coli from where it can be isolated and used for many applications, e.g., gene transfer into eukaryotic cells. Transcription-. Copying of one strand of DNA into a complementary RNA sequence by the enzyme RNA polymerase. 

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