Variable-temperature magnetic circular

Variable-Temperature Magnetic Circular Dichroism Studies of Metalloproteins... [Pg.326]

Probing Metalloproteins Electronic absorption spectroscopy of copper proteins, 226, 1 electronic absorption spectroscopy of nonheme iron proteins, 226, 33 cobalt as probe and label of proteins, 226, 52 biochemical and spectroscopic probes of mercury(ii) coordination environments in proteins, 226, 71 low-temperature optical spectroscopy metalloprotein structure and dynamics, 226, 97 nanosecond transient absorption spectroscopy, 226, 119 nanosecond time-resolved absorption and polarization dichroism spectroscopies, 226, 147 real-time spectroscopic techniques for probing conformational dynamics of heme proteins, 226, 177 variable-temperature magnetic circular dichroism, 226, 199 linear dichroism, 226, 232 infrared spectroscopy, 226, 259 Fourier transform infrared spectroscopy, 226, 289 infrared circular dichroism, 226, 306 Raman and resonance Raman spectroscopy, 226, 319 protein structure from ultraviolet resonance Raman spectroscopy, 226, 374 single-crystal micro-Raman spectroscopy, 226, 397 nanosecond time-resolved resonance Raman spectroscopy, 226, 409 techniques for obtaining resonance Raman spectra of metalloproteins, 226, 431 Raman optical activity, 226, 470 surface-enhanced resonance Raman scattering, 226, 482 luminescence... [Pg.457]

Variable temperature magnetic circular dichroism (MCD) studies have revealed similar electronic and magnetic properties for the nickel centres in Jack bean and Klebsiella aerogenes urease. Native and 2-mercaptoethanol and acetohydroxamic inhibited forms of both enzymes have been investigated and in each case the energy of the temperature dependent MCD for the nickel(II) d-d transitions indicates octahedral coordination with mainly oxygen donors. [Pg.112]

Thomson AJ, Cheesman MR, George SJ (1993) Variable-temperature magnetic circular... [Pg.160]

Recent variable temperature magnetic circular dichroism studies by Foote et al. [219] have shown that the Fe =0 myoglobin derivative exists in two forms, depending on the pH. The observed spectral differences were tentatively ascribed to deprotonation of the proximal histidine at higher pH. [Pg.35]

Thomson, A.J., Cheesman, M.R., George, S.J. Variable-temperature magnetic circular dichroism. Methods Enzymol. 226, 199-232 (1993)... [Pg.416]

Yoon J, Mirica EM, Stack TDP, Solomon El. Variable-temperature, variable-field magnetic circular dichroism studies of tris-hydroxy-and ti(3)-oxo-bridged trinuclear Cu(II) complexes Evaluation of proposed structures of the native intermediate of the multicopper oxidases. J. Am. Chem. Soc. 2005 127 13680-13693. [Pg.1403]

Polarized Absorption and Variable Temperature, Variable Field Magnetic Circular Dichroism 698... [Pg.691]

In order to understand the charge transfer features of the Blue Copper site, the variable-temperature optical absorption, room-temperature circular dichroism, and magnetic circular dichroism spectra of plastocyanin, stellacyanin, and azurin were studied355. As can be seen for plastocyanin in Fig. 12, the relative intensities (and signs, in the case of CD and MCD) of these transitions vary among the different types of spectra. This is a result of the difference in selection rules for absorption, CD, and MCD spectra, as mentioned in the Introduction. A careful comparison of the three types of spectra and the absorption bandshape temperature dependence (see moment analysis in Ref. 35, pp. 176-177)... [Pg.17]