Valentine bean

One of the earliest important observations on the time of production of a GA-like substance was made by Mitchell et al. [24] on the Black Valentine bean during fruit development. While pod development began shortly after pollination, the GA-like growth factor was barely detectable until 4 days after pollination. The growth factor was at the peak of its activity when the seed began to grow in size. 

Mego, j. L., and Jagendorf, A. T. (1961). Effect of light on growth of Black Valentine bean plastids. Biochim. Biophys. Acta 53, 237-254. 

A sample of green bean seeds (Black Valentine) was extracted with cold ether to remove fats, and then extracted with hot methanol and analyzed by paper chromatography. Raffinose was found besides other sugars. Analysis of an aliquot by an ion exchange procedure showed the raffinose content to be 0.40 mg., present in the fraction between approximately 80 and 180 ml. of the eluate. Separation and recovery of 1 mg. each of sucrose, raffinose, stachyose, and fructose in a mixture of pure sugars were checked by the same procedure and found to show a recovery of 1.02 mg. for raffinose. 

---