UV Spectra Library

The library presented in this chapter includes the spectra of 115 organic and mineral compounds of 11 substance groups. All spectra are acquired following the traceability procedure explained hereafter. In contrast to the previous libraries, the spectra are presented as the variation of absorbance with wavelength, and not the molar absorptivity. A table groups all useful information, including the concentration of the sample solution. This choice of presentation allows users to rapidly show the applicability of UV-visible spectrophotometry for their own applications. 

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Herzler, M., Herre, S., Pragst, F. Selectivity of substance identification by HPLC DAD in toxicological analysis using a UV spectra library of 2682 compounds. J. Anal. Toxicol. 27, 233-242 (2003)... 

The first group, SF>4, is composed of samples with UV spectra showing specific absorption peaks or shoulders revealing the presence of major components. In this case, the comparison of UV spectrum of a sample to a UV spectra library may allow the identification of pollutant. 

The most widely used detection methods in HPLC analysis of anilines and phenols are UV (especially diode array) and electrochemical detection (ED). UV detectors provide very good signal stability and in the case of diode-array detectors they can be used for analyte tentative confirmation purposes using UV spectra libraries. Electrochemical detectors are more sensitive than the UV detectors however, their performance is highly dependent on the type of samples analyzed. Components from dirty samples are deposited on the electrochemical cell and the detector sensitivity is rapidly decreased. ° ° The heterocyclic amines can be measured with UV, electrochemical, and fluorescence detectors. 

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