Ultrahigh-pressure liquid chromatography UHPLC

Most frequently, high-pressure liquid chromatography (HPLC) is used in LC-MS work, while more recently ultrahigh-pressure liquid chromatography (UHPLC or ultra performance liquid chromatography, UPLC, as it is termed by one manufacturer) has come into use. For very low saiiple amounts, nanoLC, i.e., capillary LC, can directly be interfaced to nanoESI. 

It can be concluded that applications of UHPLC techniques has been increasing each year. Impressive technological advances in the UHPLC have made it possible to simultaneously determinate many polyphenolic compounds. UHPLC provides high speed and high sensitivity and consumes less mobile phase. Moreover, the combination of ultrahigh pressure liquid chromatography with mass spectrometry greatly increases the sensitivity and selectivity of the quantitative analysis of polyphenolic compounds in complex matrixes. UHPLC-Q-TOE/MS and TOF/MS techniques enable the identification of new polyphenolic compounds in plants. 

While HPLC has been the standard analytical tool for protein characterization for many years, ultrahigh performance liquid chromatography (UHPLC), sometimes referred to as ultrahigh pressure liquid chromatography, has the potential to increase resolution and efficiency compared to conventional HPLC and, thus, facilitate improved protein characterization. Like conventional HPLC, UHPLC can provide... 

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