Ultrahigh-performance liquid chromatography

Granado-Lorencio, F. Flerrero-Barbudo, C. Blanco-Navarro, 1. Perez-Sacristan, B. 2010. Suitability of ultrahigh-performance liquid chromatography for the determination of fat-soluble nutritional status (vitamins A, E, D, and individual carotenoids). Anal. Bioanal. Chem. 397 1389-1393. 

A much more sensitive technique than NMR is MS detection combined with various separation techniques (e.g., high-performance liquid chromatography, HPLC ultrahigh-performance liquid chromatography, UHPLC capillary electrophoresis, CE gas chromatography, GC) that allow previous separation of metabolites. The application of MS enables analysis of large number of metabolites based on their interactions with the separation system and, above all, on the basis of their molecular weights. 

Yang, L., Yu, W., Yan, X., and Deng, C. 2012. Decyl-perfluorinated magnetic meso-porous microspheres for extraction and analysis perfluorinated compounds in water using ultrahigh-performance liquid chromatography-mass spectrometry. J. Sep. Sci. 35 2629-2636. 

Medina-Remdn, A., Tulipani, S. Rotches-Ribalta, M., Mata-Bilbao, M.D.L., Andres-Lacueva, C., and Lamuela-Raventos, R.M. 2011. A fast method coupling ultrahigh performance liquid chromatography with diode array detection for flavonoid quantification in citrus fruit extracts. J. Agric. Food Chem. 59 6353-6559. 

With the advent of fast analytical methods (fast gas chromatography (GC), capillary electrophoresis (CE), ultrahigh performance liquid chromatography (UHPLC)) [27-29], it appears that the extraction methods are the bottleneck for most of the analytical processes. Thus, there is a need for appropriate fast and efficient extractimi steps for plant samples. 

Recendy several studies have reported the use of analytical columns packed with sub-2 pm size particles (11, 12, 14, 15). These columns are especially designed for use with ultrahigh-performance liquid chromatography (UHPLC). The smaller particles are more efficient because they can be used at higher linear velocities, which provides both better resolution and shorter analysis times. UHPLC methods have been reported using isocratic and gradient elutions, a variety of mobile phases, and aqueous buffers with pH ranging from 2.6 to 8.6 to ensure optimum ionization and intensities for specific mass spectrometer ionization sources (14). 

Liu Y, Zhang J, Xu X, Zhao MK, Andrews AM, Weber SG. Capillary ultrahigh performance liquid chromatography with elevated temperature for sub-one minute separations of basal serotonin in submicroliter brain microdialysate samples. Anal Chem 2010 82 9611-6. 

Ultrahigh performance liquid chromatography was used for the determination of imidazole, 4-methylimidazole, and 2-methylim-... 

Practical Aspects of Ultrahigh Performance Liquid Chromatography... 

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