Ultra-violet microscope

B.K. Johnson, An ultra-violet microscope for the examination of opaque objects. J. Sci. Instrum. 11, 384-394 (1934)... 

F.F. Lukas, The architecture of living cells - recent advances in methods of biological research - optical sectioning with the ultra-violet microscope. Proc. Natl. Acad. Sd. 16, 599-607 (1930)... 

Procedure The fluorescence of the objects, which lie on the object glasses (slices) and excited by ultra-violet (360-380 nm) or violet (400-420 nm) light, may be seen in luminescent microscope with multiplication of objectives x 10,20,40 or with water immersion x 85, or with immersion oil x 60 and 85. ... 

Spectroscopic techniques, such as ultra-violet (9), Infrared (25), Nuclear Magnetic Resonance (24), and Fluorescence spectroscopies (5-8), constitute direct probes of specific events occurring at the molecular scale. When a quantitative interpretation is possible, spectroscopy provides very detailed microscopic information. Unfortunately however, the interpretation of spectra in terms of molecular events is often complex. Yet another approach that probes events at the molecular scale involves the use of tracers, such as chromophores (1-225). Again, the complexity of the tracer imposes limitations on the extent to which the data can be interpreted quantitatively. 

Electron - microscopic photographs of dyed and undyed PCA fibres before and after ultra-violet irradiation were obtained to clarify possible effect of bis-aroilenbenzimidazole derivatives on supermolecular structure of PCA fibres by the method of raster electronic microscopy (REM) with increase of 12000 times. Any specific details of the structure were not observed in the initial sample. After irradiation the initial sample undergoes great... 

A. Cosslett, Some applications of the ultra-violet and interference microscopes in electron microscopy. J. R. Microsc. Soc. 79, 263-271 (1960)... 

Preliminary experimentation was carried out to investigate the reasons for lack of contrast and subsequent discolouration, on commercially available keyboards supplied by NMB Minebea UK Ltd. Keycaps were tested for durability using a specialised wear test, accelerated ultra-violet weathering test and accelerated chemical resistance test, as described below. Microscopic examination of the samples was also carried out. 

According to J. Amann,7 brown soln. of iodine contain ultra-microscopic particles, while the violet soln. contain none. The violet-red soln. in benzene, toluene, and xylene are photosensitive in that clouds of ultramicroscopic particles are formed in white light, and the soln. rapidly turn brown. The soln. return to their original state in darkness. In some cases, iodine soln. are true soln. either of free iodine or of an addition compound and in other cases they contain free colloidal iodine or of a polymerized addition compound. In some cases ultra-microscopic suspended particles of iodine are present. 

This is a complex compound of aluminium and sodium silicate and sodium sulphide. The typical and most common ultramarine is blue ultramarine, which forms a very fine powder (microscopically crystalline) of a pure blue, reddish blue or greenish blue colour according to the proportions of the components and the method of preparation ultramarines of other tints, especially green and violet ultramarines, are also made. Ultra-marine may be adulterated with gypsum, chalk, clay, heavy spar, zinc white and magnesium carbonate and may also be mixed with glycerine and glucose. 

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