Ultra-thin-layer gel electrophoresis

The aim of this chapter is to cover the theoretical and practical aspects of capillary gel electrophoresis. It also provides an overview of the key application areas of nucleic acid, protein, and complex carbohydrate analysis, affinity-based methodologies, as well as related microseparation methods such as ultra-thin-layer gel electrophoresis and electric field-mediated separations on microchips. It also gives the reader a better understanding of how to utilize this technology, and determine which actual method will provide appropriate technical solutions to problems that may have be perceived as more fundamental. Micropreparative aspects and applications are discussed in Chapter 12. 

Ultra-thin-layer gel electrophoresis is a combination of slab-gel electrophoresis and capillary gel electrophoresis [130], providing a multilane separation plat-... 

In addition to the use of conventional preseparation labeling, ultra-thin-layer gel electrophoresis systems readily accommodate fluorophore labeling dur-... 

Automated ultra-thin-layer gel electrophoresis was applied for large-scale analysis of SDS-protein complexes and for molecular-mass estimation of the proteins in the sample [12], Figure 13 compares the separations of two differ-... 

Guttman, A., Z. Csapo, and D. Robbins. 2002. Rapid two-dimensional analysis of proteins by ultra-thin layer gel electrophoresis. Proteomics 2 469-74. 

Gll. GSrg, A., Postel, W., and Westermeier, R., Ultra-thin-layer horizontal electrophoresis, isoelectric focusing and protein mapping in polyacrylamide gels on cellophane. In Electrophoresis 79 (B. J. Radola, ed.), pp. 67-78. Walter de Gruyter, Berlin, 1980. 

Isoelectric focusing and electrophoresis were used for protein mapping and to study protein extraction in horizontal ultra-thin-layer format [138], The 0.12-0.36-mm-thick polyacrylamide gel layer was deposited onto tiny glass plates (e.g., microscope slides). The method enabled the analysis of 1-ng tissue culture specimens. Ultra-thin-layer polyacrylamide isoelectric focusing gel was also employed in two-dimensional analysis of plant and fungal proteins. Marlow et al. [139] reported on the use of 0.2-mm semirigid backing (polyester)-supported... 

Szoke M, Sasvari-Szelely M, Guttman A. Ultra-thin-layer agarose gel electrophoresis, I. Effect of the gel concentration and temperature on the separation of DNA fragments. J Chromatog A 1999 830 465-71. 

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