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Ubiquitin vinyl sulfone

A class of DUBs only identified since 2002 is the OTU (ovarian tumor protein) DUB class. The OTU domain was originally identified in an ovarian tumor protein from Drosophila mdanogaster, and over 100 proteins from organisms ranging from bacteria to humans are annotated as having an OTU domain. The members of this protein superfamily were annotated as cysteine proteases, but no specific function had been demonstrated for any of these proteins. The first hint of a role for OTU proteins in the ubiquitin pathway was afforded by the observation that an OTU-domain-containing protein, HSPC263, reacted with ubiquitin vinyl sulfone (an active-site-directed irreversible inhibitor of DUBs) [41]. [Pg.197]

The most promising tools developed for this sort of analysis are active-site-directed irreversible inhibitors of DUBs. These inhibitors are ubiquitin or ubiquitin-like proteins chemically modified at the C-terminus by an electrophilic moiety such as a Michael acceptor or alkyl halide. The modified ubiquitin can be incubated with a purified DUB or a cell lysate containing DUB activity. Ubiquitin vinyl sul-fone (UbVS) is one such irreversible inhibitor because the vinyl sulfone moiety reacts with the active-site cysteine of the DUB, forming a thioether linkage. The covalent adduct is stable and can be detected in a variety of ways. Labeling of DUBs is specific, as only a DUB active-site cysteine will efficiently react with the vinyl sulfone moiety. [Pg.209]

This labeling has been used with success in yeast where 6 of the 17 known DUBs were labeled with UbVS [115]. Incomplete labeling likely results from DUBs that do not act on mono-ubiquitin or where the UbVS could not access the active site. The labeling has also been used with great success in mammalian cell lysates to identify novel ubiquitin DUBs [41]. A novel deneddylating enzyme and a novel DUB that acts on autophagy-related UbL proteins have also been identified using vinyl sulfone labeled probes [26, 37, 116]. [Pg.209]

This ubiquitin intein system can also be utilized to make a DUB substrate rather than inhibitors by attaching a C-terminal fluorescent tag such as 7-amidomethylcoumarin (AMC) instead of vinyl sulfone. DUBs cleave the ubiquitin derivative and release the fluorescent tag, a process that can be followed fluoromet-rically. Eluorometric assays can then be used to determine a particular DUB s preferred substrate or to quantitate DUB activity in crude lysates. AMC substrates... [Pg.209]


See other pages where Ubiquitin vinyl sulfone is mentioned: [Pg.304]    [Pg.304]    [Pg.209]   
See also in sourсe #XX -- [ Pg.209 ]




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Ubiquitin, ubiquitination

Ubiquitination

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