Tumors cell attachment assay

Braun AG, Nichinson BB, Horowitz PB. 1982. Inhibition of tumor cell attachment to concanavalin A-coated surfaces as an assay for teratogenic agents Approaches to validation. Teratogenesis Carcinog Mutagen 2(3-4) 343-354. 

Tumor cells that have the ability to attach to some defined substrates with the same apparent efficiency, as measured by the adhesion assays described above, may nonetheless adhere to the different substrates not with the same strength. Therefore, even though each substrate appears to be able to support cell adhesion, the tenaciousness characterizing each interaction may be different, and it may have an influence on the metastatic ability of tumor cells (Leung-Tack et al., 1988). 

Although all the methods mentioned above can generate reproducibly sized tumor spheroids, the one that we recommend is the method that uses round-bottom ultralow attachment 96-well plates (11). This method is the least time consuming (e.g., plates do not require any coating), reproducible (one centrally located spheroid/well is formed), and suitable for automated imaging. Thus it satisfies important requirements of cell-based HT assays. 

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