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Cell attachment assay

Braun AG, Nichinson BB, Horowitz PB. 1982. Inhibition of tumor cell attachment to concanavalin A-coated surfaces as an assay for teratogenic agents Approaches to validation. Teratogenesis Carcinog Mutagen 2(3-4) 343-354. [Pg.168]

If the teratocarcinoma cells become confluent they will begin to differentiate into many different cells types, but this process is not so dramatic as the differentiation which occurs if cells isolated by method 2 are transferred to a vessel without a feeder layer. Then the cells attach very poorly and form clumps in suspension. These clumps remain healthy and quickly differentiate (Evans, 1972) to form an outer layer of endoderm cells. The presence of endoderm can be shown by assaying for the serine protease plasminogen activator which is a marker typical of endoderm cells (Strickland et al., 1976). [Pg.306]

Assay of exocytosis for cells attached to culture plates... [Pg.265]

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