Tsien studies

Kao and Tsien studied the Ca +-binding kinetics of fura-2 and azo-1 by temperature-jump relaxation methods. In 140 mM KCl at 20°C, the respective association and dissociation rate constants for fura-2 were 6x10 M s and 97 s these kinetic properties were insensitive to hydrogen ion concentration over the pH range from 7.4 to 8.4. Azo-1 was studied in 140 mM KCl At 10°C, azo-... 

Cybernetics. The scientific study of those methods of control and communication which are common to living organisms and machines, esp as applied to the analysis of the operations of devices such as computers Refs 1) C.L. Barnhart, ed, "The American College Dictionary , Random House, NY (1952), p 301-L 2) H.S. Tsien, "Engineering... 

Tsien RW, Weingart R Inotropic effect of cyclic AMP in calf ventricular muscle studied by a cut end method. J Physiol (Lond) 1976 260 117-141. 

Mee CJ, Tomlinson SR, Perestenko PV et al (2004) Latrophilin is required for toxicity of black widow spider venom in Caenorhabditis elegans. Biochem J 378 185-91 Meiri H, Erulkar SD, Lerman T et al (1981) The action of the sodium ionophore, monensin, or transmitter release at the frog neuromuscular junction. Brain Res 204 204-8 Meldolesi J, Huttner WB, Tsien RY et al (1984) Free cytoplasmic Ca2+ and neurotransmitter release studies on PCI 2 cells and synaptosomes exposed to a-latrotoxin. Proc Natl Acad Sci US A 81 6204... 

Harata NC, Choi S, Pyle JL, Aravanis AM, Tsien RW. Frequency-dependent kinetics and prevalence of kiss-and-run and reuse at hippocampal synapses studied with novel quenching methods. Neuron 2006 49 243-256. 

The two major sources that contribute to a rise in [Ca2+]j are the release of intracellular Ca + from the SR and the influx of extracellular Ca + (van Breemen and Saida, 1989 Tsien and Tsien, 1990 Missiaen et al., 1992 Pozzan et al., 1994). Therefore, two important questions are (1) does genistein-sensitive tyrosine kinase activity participate in regulatory mechanisms that couple receptor activation and release of intracellular Ca +, and (2) does tyrosine kinase activity participate in mechanisms that couple receptor activation to influx of extracellular Ca2+ To address these questions, we studied the effects of genistein on the Ca + response evoked by AVP or Et in the presence or absence of extracellular Ca2+. Ca + responses elicited in the absence of extracellular Ca + (0.5 mM EGTA and no added Ca +) purportedly reflect release of intracellular Ca2+ from vesicular storage sites such as the SR. Therefore, as amply documented in many studies, comparison of Ca + response elicited in the presence and absence of extracellular Ca + allows for assessment of the relative contribution of influx and release pathways to the evoked change in [Ca +Jj. 

Benham, C.D., Hess, P., and Tsien, R.W. (1987) Two types of calcium channels in single smooth muscle cells from rabbit ear artery studied with whole-cell and single-channel recordings. Circulation Research, 62 110-116... 

The first model based on CICR was proposed by Kuba Takeshita (1981) for Ca oscillations in sympathetic neurons (see Friel Tsien, 1992, for a recent study of Ca oscillations in this type of cell) the role of IP3, unknown at that time, was not taken into account. Dupont and Goldbeter, in collaboration with Berridge, later considered the model shown in fig. 9.4c, of a more general nature, where CICR is initiated by the IP3-mediated build up of cytosolic Ca (Dupont Goldbeter, 1989 ... 

Fluorimetric methods have proven useful for the assay of metal ions in solution (i) for example, in vivo studies of calcium-selective fluorescence probes have been reported by Tsien (2). Most such analytical methods reported to date involve complexation of metal ions with aromatic heterocyclic ligands ("intrinsic" fluoroionophores). In 1977, Sousa described the synthesis of naphthalene-crown ether probes (Figure 1) in which the fluorophore 7C-system is insulated from the... 

Osamu Shimomura (Japan), Martin Chalfie (United States), and Roger Y. Tsien (United States) for the discovery and development of the green fluorescent protein, GFR These three scientists received the prize for their work with a protein simply called green fluorescent protein, or GFR This protein was first observed way back in 1962 in a jellyfish. It eventually became a very important molecule in the biological sciences, as clever researchers have found ways to use GFP to study otherwise invisible chemical processes. Shimomura, Chalfie, and Tsien were at the forefront of the major discoveries that led to the understanding of GFP that researchers possess today. 

Tsien, H. C., Mosharrafa, E. T., Hickman, D. D., Hagen, E. W., Schachtele, C. F., Anderson, D. L. Studies with bacteriophage 029 and its infectious DNA. Proceedings Summer School on Uptake of Informational Molecules. Mol. Belgium 1970. In press. 

Heim, R., Prasher, D.C., and Tsien, R.Y., Wavelength mutations and posttranslational autoxidation of green fluorescent protein, Proc. Natl Acad. Sci. USA, 91,12501-12504,1994 Sieghbahn, P.E.M., Wirstam, M., and Zimmer, M., Theoretical study of the mechanism of peptide ring formation in green fluorescent protein, 81,169-186, 2001. 

---