Tsien, Roger

I am grateful to Dr Oliver Griesbeck (Max Planck Institute of Neurobiology Am Klopferspitz, Martinsried, Germany) and Prof. Roger Tsien (Howard Hughes Medical Institute, University of California, San Diego, USA) for the... 

SOURCE Modified version of figure supplied by Roger Tsien. 

Roger Y. Tsien Fluorescent indicators/sensors for intracellular calcium... 

American chemist Roger Y. Tsien is born on February 1 in New York, New York. 

Martin Chalfie and Roger Y. Tsien independently perform the research that leads to the Nobel Prize in chemistry. 

I would like to thank all my coworkers over the years in the Tsien and Ellisman labs who have contributed to the development of the biarsenical-tetracysteine method, particularly Roger Tsien for devising the original concepts and for continual input into their improvement. 

Martin Chaifie, Osamu Shimomura, Roger Y. Tsien 1954 Linus Pauling... 

This approach to the imaging of intracellular Ca " flux was initiated by the work of Dr. Roger Tsien. At the University of California at Berkeley, he developed the Ca -selective chelator, BAPTA (2) and it s initial fluorescent derivatives. With the invention of quin-2 in 1980 (J) and Ae subsequent indicators, fura-2, indo-1 (4), fluo-3 and rhod-2 (5), researchers in the biological sciences have begun to define the complex role of Ca " in living cells (6). 

Osamu Shimomura (Japan), Martin Chalfie (United States), and Roger Y. Tsien (United States) for the discovery and development of the green fluorescent protein, GFR These three scientists received the prize for their work with a protein simply called green fluorescent protein, or GFR This protein was first observed way back in 1962 in a jellyfish. It eventually became a very important molecule in the biological sciences, as clever researchers have found ways to use GFP to study otherwise invisible chemical processes. Shimomura, Chalfie, and Tsien were at the forefront of the major discoveries that led to the understanding of GFP that researchers possess today. 

It can be determined if the protein is associated with the cell membrane, if it is ever transferred from one cell to another, what its fate is during embryonic development, and so on. The key is that the chromophore forms with no assistance, and so the fluorescence develops in almost any environment. In fact, green fluorescent animals have been prepared by transgenically incorporating GFP into the animal s genome. GFP-based assays for various cellular processes have been developed because the fluorescence is so easy to detect. Entire volumes have been written on the diverse ways this structure from a jellyfish has been put to good use. The 2008 Nobel Prize in Chemistry was awarded to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their discovery and development of GFP. 

Green fluorescent protein (GFP), first isolated from biolumlnescent jellyfish, Is a protein containing 238 amino acid residues. The discovery of GFP has revolutionized the field of fluorescence microscopy, which enables biochemists to monitor the biosynthesis of proteins. The 2008 Nobel Prize in Chemistry was awarded to Martin Chalfie, Osamu Shimomura, and Roger Tsien for the discovery and development of GFP. The structural subunit of GFP responsible for fluorescence, called the fluorophore, results when three amino acid residues undergo cyclization. Identify the three amino acids that go into the biosynthesis of this fluorophore ... 

The 2008 Nobel Prize in Chemistry was awarded to Osamu Shimomura, Martin Chalfie, and Roger Tsien for their work in the development of GFP. 

Acknowledgements We thank Elke Haustein for helpful discussion and proofreading the manuscript. Plasmids encoding the fluorescent proteins mRFPl and tdimer2(12) were kindly provided by Roger Y. Tsien (University of California at San Diego). 

I ll begin by telling you a bit about fluorescent proteins and how they ve revolutionized experimental biology. A key contributor to this area is Professor Roger Y. Tsien of University of California, San Diego (UCSD) As I m sure you know. Professor Tsien, along with Martin Chalfie and Osamu Shimomura, received the 2008 Nobel Prize in Chemistry for the discovery and development of a fluorescent protein called green fluorescent protein (GFP). You should definitely look up his Nobel Prize lecture and maybe even make an effort to visit his laboratory. 

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