Trypsinization mouse embryo cells

To find whether the reduced foci formation was due to inhibition of MSV (M) replication, the effect of these compounds on virus growth was studied. Secondary mouse embryo cell cultures were infected with MSV (M) at the rate of 0.03 competent MSV infectious units per cell and treated with the compounds at different doses after the infection by the focus assay in the presence of an optimal amount of MLV (M) (1.01 10s Leukemia Vims Helper Units) according to Hirschman etal.33 Similarly treated uninfected cultures were trypsinized at the same time, and cells were counted. 

Inbar and coworkers689 determined that [3H]-acetylated lectin did not bind to the surface of normal cell-lines (hamster and rat embryo, and mouse 3T3 cells) or to the transformed counterparts of these cells. Trypsinization did not alter these results. The authors concluded688 that L-fucosyl residues were not exposed on the cell surface. In view of the specificity studies of Kabat and coworkers,200,686 this interpretation may be incorrect L-fucosyl residues may be rendered sterically inaccessible by glycosylation of neighboring sugar residues. 

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