Tris-buffered saline, solution preparation

For plasminogen-deficient fibrinogen from blood plasma, the anticoagulated blood was centrifuged and the plasma was frozen and washed with saline solution. Treated with charcoal and freeze-thawed. Dialysed versus Tris/NaCl buffer. [Maxwell and Nikel Biochemical Preparations 12 16 1968]. 

A 10 mmol L stock coelenterazine solution was prepared by dissolving coelenterazine (Nanolight Technology, Prolume Ltd. Pinetop, AZ, USA) in methanol for use at a final concentration of 10 /tmol L". All coelenterazine solutions were stored at -20 °C and working solutions were kept on ice in the dark during preparation. Diluent buffers comprised distilled water (dH20), Phosphate buffered saline (PBS), Buffer A (10 mmol L Tris [pH 7.8], 1 mmol L EDTA, 0.6 mol L NaCl), 7H9 medium supplemented with Tween-80 with or without 10% OADC (oleic acid, albumin, dextrose, catalase), Luria-Bertani (LB) broth with or... 

The pH of a tris buffer solution prepared as described above is a function of temperature and salinity and can be estimated from the following equation Almgren et oL, 1975) ... 

Oligonucleotide solutions are prepared in TE buffer pH 8 (0.1 M Tris-HCl buffer solution, ImM in EDTA). Aliquots are prepared and maintained at -20°C. Working solutions were conserved at 4°C. Hybridisation takes place in a 2 x SSC (saline sodium citrate, 30 mM sodium citrate buffer with 300 mM sodium chloride and pH 7.0) buffer containing 50% of formamide. 

P 82] Dilution-type mixing was accomplished with the fluorescent dyes acridine orange (0.01% solution in 20 mM in TE buffer see below) or trypan blue (prepared in 0.85% saline) contacted with buffer solution (TE buffer 10 mmol f4 Tris-HCl, pH 7.4, 1 mmol 1 1 EDTA, pH 8.0) [164]. Images were taken by a laser scanning confocal microscope. Profiling data analysis was employed along detection lines. 

Potassium dihydrogen citrate 0.05 molal solution (pH=3.776 at 25 °C) is used for calibration purposes because it exhibits better stability than primary pH reference buffer solutions of tartrate or phthalate [44, 45]. The saline sodium citrate buffer (SSC) prepared from tri-sodium citrate and sodium chloride (pH=7.0) is applied in biochemistry. Citric buffers with different HjCit Na3Cit ratios are clinically effective, for example in reducing gastric acidity [46-48]. Compositions of buffers and corresponding pH values are presented in Table 3.7. 

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