Triglyceride ultrafiltration

The separation,purification and concentration of a thermosensitive bioactive compound from a lysate has been carried out combining UF, ion exchange and RO with significant cost reduction and productivity increase. Enzyme membrane reactors have been used for triglyceride enzymatic hydrolysis and product separation. Thermophi1ic,thermostable enzyme ultrafiltration membrane have been prepared, and used in high temperature lactose hydrolysis. 

Fig. 2. Light scattering intensity (LSI arbitrary units) of serum lipoproteins of different size after loading with monoglycerides (MG), free fatty acids (FFA), a 2 1 mixture of free fatty acids with monoglycerides or triglycerides (TG). All fats had the fatty acid composition of safflower seed oil the monoglycerides had equilibrium isomeric composition, and fats were fed at 1 g/kg body weight. Lipoproteins were separated by ultrafiltration into fractions of different size [diameter (d) > 400 nm, 100 < d < 400 nm, and d < 100 nm].

Li-Hua, C., Ya-Fang, C., Shih-Yang, Y., Junghui, C. (2009). Ultrafiltration of triglyceride from biodiesel using the phase diagram of oil—FAME—MeOH. Journal of Membrane Science, 330, 156—165. 

Various membrane reactors have been proposed to allow recovery of products from microemulsions and permit to reuse the biocatalyst in various enzymatic processes such as hydrolysis of triglycerides [124,182], synthesis of esters [183,184], and peptide synthesis [65,185,186]. These reactors take advantage of the potential of synthetic semipermeable membranes, that usually are ultrafiltration membranes, to retain enzymes on the membrane and to recover products on the permeate side. The productivities as well as the enzyme stability in such reactors are usually high. 

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