Tresyl chloride

Figure 14.14 Additional hydroxyl-particle activation methods include bis-epoxide modification, tosyl activation, and tresyl activation methods. The tosyl chloride and tresyl chloride activation procedures must be done in dry organic solvent, but the coupling of an amine-containing ligand can be done in either organic solvent or aqueous buffer.

Tresyl chloride/tosyl chloride method, for covalent ligand immobilization, 6 396t Tretinoin, 25 789... 

Cyanogen bromide method A,A -Carbonyl diimidazole method Divinylsulfone method Epoxy (bisoxirane) method Ethyldimethylaminopropyl carbodiimide method Eluoromethylpyridinium toluenesulfonate method A-hydroxysuccinimide ester method Schiff base (reductive amination) method Tresyl chloride/tosyl chloride method Sulfhydryls Azalactone method (for azalactone supports)... 

Tresyl chloride/tosyl chloride method Sulfhydryl groups ... 

Reaction of surface hydroxyl groups with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) yields sulfonate esters which in a subsequent step can be reacted with amino- or mercapto-modihed biomolecules for covalent immobilization... 

McConway MG and Chapman RS. Application of solid-phase antibodies to radioimmunoassay evaluation of two pol3mieric microparticles, dynospheres and nylon, activated by carbonyldiimidazole or tresyl chloride. J. Immunol. Methods 1986 95 259-266. 

Epoxide Tresyl chloride Carbonyldiimidazole Thiol/disulfide exchange... 

A simple method for the activation of hydroxyl group carrying supports such as agarose, cellulose, diol-silica, glycophase-glass or hydroxyalkyl methacrylate copolymer with 2,2,2-trifluoroethanesulfonyl chloride (tresyl chloride) was introduced by Nilsson and Mosbach [108] ... 

The advantage of tresyl chloride is that it is more reactive than the previously described tosyl chloride [109], and therefore it allows efficient coupling under very mild conditions, and to a number of different supports including those used for HPLC. 

Alkyl N-tresylarylsulfonimides can be obtained by the reaction of (2) with tresyl chloride (2,2,2-trifluoroethanesulfonyl chloride). ... 

Figure 14-1 Functionalization and activation of electrode materials, a) silanization of supports bearing hydroxylic groups b) reaction with cyanuric chloride c) activation with cyanogen bromide d) binding via amide bonds after activation with 1,1-carbonyldiimidazole e) activation with good leaving groups such as tresyl chloride f) formation of Schiff bases between aldehyde groups on the support and amino groups at the biomolecule g) activation of carboxylic groups with carbodiimides or h) via the acid chlorides.

Other activation methods are performed with carbonyldiimidazole or tresyl chloride (14) both jdeld uncharged materials. Coupling with carbonyldiimidazole (CDI) is very rapid, but the bond formed, a substituted carbamate, is not as stable as the secondary amine formed with the slower tresyl chloride. 

For initial activation with CNBr, tresyl chloride etc. Proteins, peptides... 

K. Nilsson, K. Mosbach, Tresyl chloride-activated supports for enzyme immobilization, Methods Enzymol. 135 (1987), 65-78. 

H. P. Jennissen, Analysis of affinity supports by C CP/MAS NMR spectroscopy application to carbonyl-diimidazole- and novel tresyl chloride-synthesized agarose and silica gels. 

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