Transposons Vector

Herrero, M., de Lorenzo, V. Timmis, K. N. (1990). Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria.Journal of Bacteriology, 172,6557-67. 

Polissi, A., Bertoni, G., Acquati, F. Deho, G. (1992). Cloning and transposon vectors... 

Infection) transduction Km Suicide transposon vector Polissi et al. (1992)... 

Zayed H, Izsvak Z, Walisko O, Ivies Z (2004) Development of hyperactive Sleeping Beauty transposon vectors by mutational analysis. Mol Ther 9 292-304. 

Handler A. and Harrell R.A. 1999. Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. Insect Mol Biol 8 449—458. 

Tamura, T., Thibert, C., Royer, C. et al. 2000. Germline transformation of the silkworm Bombyx mori L. using apiggyBac transposon-derived vector. Nat. Biotechnol., 18(1) 81—84. 

The target gene is markedwith yellow, transposon is blue and the shuttle vector is indicated with a circle. The vector delivering the transposon will be eliminated after the random insertion of the transposable element. Insertion of the transposon inactivates the target gene function this can be corrected via introduction of the gene on a vector (complementation). 

Jones, J. D. G., Shlumukov, L., Garland, E., English, J., Scofield, S. R., Bishop, G. J. and Harrison, K. 1992. Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in plants. Transgenic Research, 1 285-297. 

Salmonella typhimurium Transposons Expression of enzyme capable of converting subsequently administered prodrug to active form Nonviral gene vector Cytotoxic... 

Delivered to target cells by plasmid. retroviral vector or transposon... 

Gonzalez-Estevez, C., Momose, T., Gehring, W.J. and Salo, E. (2003) Transgenic planarian lines obtained by electroporation using transposon-derived vectors and an eye-specific gfp marker. Proceedings of the National Academy of Sciences USA 100, 14046-14051. 

Fig. 7.1. A planarian individual of a line transformed with a Hermes transposon-derived vector 12 months after microinjection and subsequent electroporation. Besides the transposon part, the vector contained the E(enhanced)GFP reporter gene under the control of an artificial promoter that responds to the transcription factor Pax6 (Gonzalez-Estevez et ai, 2003). Binding sites for this transcription factor are found in rhodopsin and other photoreceptor-specific genes. Reporter-gene activity was found in the eyes (arrows) of transformed individuals or in regenerated heads as a mosaic of EGFP-positive photoreceptor cells. Figure courtesy of Dr E. Salo.

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