Gene vectors

Takae S, Miyata K, Oba M, Ishii T, Nishiyama N, Itaka K, Yamasaki Y, Koyama H, Kataoka K (2008) PEG-detachable polyplex micelles based on disulfide-linked block catiomers as bioresponsive nonviral gene vectors. J Am Chem Soc 130 6001-6009... 

Furthermore, some adamantane derivatives possess special affinity to bind to DNA, thereby stabilizing it. This is an essential feature for a gene vector. Some polymers have been synthesized using adamantane derivatives, the application of which is under investigation for dmg delivery. 

IEC is also important in large-scale manufacturing of gene vectors such as plasmid DNA. In one process flow scheme, IEC and size exclusion chromatography (SEC) were used sequentially to purify the plasmid after lysis... 

For small molecules, natural products, peptides, oligonucleotides, gene vectors, recombinant proteins and monoclonal antibodies ... 

Finsinger D, Remy JS, Erbacher P, Koch C, Plank C (2000) Protective copolymers for nonviral gene vectors synthesis, vector characterization and application in gene delivery. Gene Ther 7 1183-1192... 

Meyer M, Wagner E (2006) pH-responsive shielding of non-viral gene vectors. Expert Opin Drug Deliv 3 563-571... 

Interaction of polyamine gene vectors with RNA leads to the dissociation of plasmid DNA-carrier complexes. J Gene Med 8 1416-1424... 

Harada A, Kawamura M, Matsuo T et al (2006) Synthesis and characterization of head-tail type polycation block copolymer as non-viral gene vector. Bioconjug Chem 17 3-5... 

In vitro models based on CF airway epithelium have been used widely in order to better understand the CF pathophysiology, to have a tool to study alterations in airway permeability and to assess the efficiency of gene vectors. Utilising approaches similar to those for cultivation of cells from healthy tissues, several protocols have been developed to culture airway epithelial cells harvested from CF patients [83, 84], The development of immortalised cell lines with a CF phenotype has been a significant benefit for investigators in... 

Liposome-Polycation-DNA A Nonviral Gene Vector Turned into a Potent Vaccine Carrier... 

Scholthof, H.B., Scholthof, K.-B.G., and Jackson, A.O. (1996). Plant virus gene vectors for transient expression of foreign proteins in plants. Anna. Rev. Phytopathol. 34 299-323. 

Salmonella typhimurium Transposons Expression of enzyme capable of converting subsequently administered prodrug to active form Nonviral gene vector Cytotoxic... 

National Gene Vector Labs University of Michigan 3560 E MSRB II... 

In 1991, Luger et al. revealed by X-ray analysis the crystal structure of a natural DNA-histone complex. The X-ray structure shows in atomic detail how the histone protein octamer is assembled and how the base pairs of DNA are organized into a superhelix around it [74]. Since then this protein structure with cationic amino acids on the surface has acted as a model for the rational design of dendritic polymer-based gene vectors to mimic the globular shape of the natural histone complex [75-77]. 

Hyperbranched polymers represent a compromise between the perfect structures of dendrimers and the partially degraded architecture of activated PAMAMs. In this way such polymeric architectures are the structural prerequisites implicated in the design of an efficient gene vector. Hyperbranched polymers are highly branched molecules, composed of dendritic (D), linear (L), and terminal units (T) (Fig. 5). 

Scheme 7 Synthetic pathway followed for the synthesis of PG-PEI gene vectors. Reproduced with permission from [131]. Copyright 2010 Elsevier...

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