Transgenics strain types

Wild-type nematodes (N2) can be used for RNAi. If stronger knockdown is required, an RNAi-sensitive strain such as rrf-3(pkl426) strain NL2099 can be used. The rrf-3(pkl426) strain is temperature sensitive and sterile at 25°C. Transgenic strains expressing GFP in cell types of interest see Table 1) are also available and facilitate identification of migratory cells. 

Clostridium thermoceUum demonstrated one of the fastest growth rates on crystalline cellulose by the use of a highly efficient cellulosome [54]. However, the construction of stable transgenic strains has been hmited by the lack of effective genetic tools. Tripathi etal. [55] developed positive and negative selectable markers to allow for the selection of strains that undergo allele replacement. The deletion of the Idh and pta genes, and a 2000-h adaptation, led to a 4.2-fold increase in the ethanol yield compared to wild-type cells. 

Michou AI, Santoro L, Christ M, Julhard V, Pavirani A, Mehtali M (1997) Adenovirus-mediated gene transfer Influence of transgene, mouse strain and type of immune response on persistence of transgene expression. Gene Ther 4 473 82. 

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