Clostridium thermoceUum

Lemaire, M., Miras, 1., Gounon, P., Beguin, P., 1998. Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermoceUum. Microbiology 144, 211—217. 

Clostridium thermoceUum demonstrated one of the fastest growth rates on crystalline cellulose by the use of a highly efficient cellulosome [54]. However, the construction of stable transgenic strains has been hmited by the lack of effective genetic tools. Tripathi etal. [55] developed positive and negative selectable markers to allow for the selection of strains that undergo allele replacement. The deletion of the Idh and pta genes, and a 2000-h adaptation, led to a 4.2-fold increase in the ethanol yield compared to wild-type cells. 

---