Transgenic plants peroxisomes

Mittendorf V, Robertson EJ, Leech RM, Kruger N, Steinbuchel A, Poirier Y (1998) Synthesis of medium-chain-length polyhydroxyalkanoates in Arabidopsis thaliana using intermediates of peroxisomal fatty add beta-oxidation. Proc Natl Acad Sd USA 95 13397-13402 Mittendorf V, Bongcam V, Allenbach L, Coullerez G, Martini N, Poirier Y (1999) Polyhydroxyalkanoate synthesis in transgenic plants as a new tool to study carbon flow through beta-oxidation. Plant J 20 45-55... 

Harms et al. [197] produced transgenic potato plants overexpressing a flax allene oxide synthase gene. These plants accumulated allene oxide synthase proteins in chloroplasts and accumulated jasmonic acid in levels equivalent to or higher than the wound-induced levels in control plants, but did not accumulate pin 2. The authors speculated that the reason for the lack of pin 2 expression might be involvement of a cytosolic LOX/allene oxide synthase pathway in pin 2 induction or sequestration of jasmonic acid in chloroplasts or peroxisomes in allene oxide synthase transgenic plants, but not wounded plants. Creelman and Mullet [29]... 

Ever since the first successful expression of PHA biosynthetic enzymes in plants [169], which resulted in the accumulation of small amounts (<0.1% of the DCW) of P(3HB), considerable knowledge regarding the potential of this system has been obtained. Subsequent studies showed that, when PHA accumulation is targeted in the plastids, a P(3HB) content of up to 14% of the DCW was accumulated by one of the transgenic plants [170]. Later, upon the expression of a threonine deaminase protein, in addition to enzymes of PHA biosynthesis, it was demonstrated that plants can be directed to produce a copolymer of P(3HB-co-3HV) [171]. This achievement is of considerable interest because of the poor physical properties of the P(3HB) homopolymer. Later, it was shown that the targeting of a PHA synthase from P. aeruginosa into the peroxisomes of Arabidopsis thaliana, results in the accumulation of PHAmcl [172]. These achievements show that it is indeed possible to produce various kinds of PHA homopolymers and copolymers in transgenic plants. 

Table 6.1, Fig. 6.2b) (Ono et al. 2012). OsPA03, 0sPA04, and OsPAOS have PA back-conversion activity (Ono et al. 2012). Studies on Arabidopsis and rice PAOs suggest that, even in other plants, peroxisome-localized PAOs could be predicted to have back-conversion activity. OsPAOl lacks introns, similar to that of the Arabidopsis AtPAOS. OsPAOl expression is induced by treatment with tetraamine, Spm, or T-Spm. In 7-promoter GPP-transgenic rice plants, the initially... 

Fig. 4. Modification of plant metabolic pathways for the synthesis of poly(3HAMCL) in peroxisomes. The pathways created or enhanced by the expression of transgenes (P. aeruginosa PHA synthase and C. lanceolata decanoyl-ACP thioesterase) and of mutant alleles of plant fatty acid desaturase genes are highlighted by bold arrows and the enzymes involved underlined...

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