Transfer NOE experiments

In principle, there are different ways to characterize the complexes On one hand, in favourable cases, it is possible to measure the NMR parameters of the receptor in the free and bound states, i.e., differences in chemical shift and NOE-contacts, before and after the formation of the complex. On the other hand, it is usually more feasible to observe and detect changes in the ligand NMR signals before and after the binding process. In this latter case, the use of STD (saturation transfer difference) and TR-NOE (transferred NOE) experiments is of paramount importance. 

In the transferred NOE experiment, RCIOSS is the cross-relaxation rate of the ligand weighted average over the free and bound state (Eq. 16.7). The experiment works best... 

The branched trisaccharide 1 was defined as the minimal carbohydrate epitope for effective binding to anti-GAS Abs [79]. The bound conformation of 1 in complex with the IgG mAb Strep 9 was investigated by transferred NOE experiments and found to correspond to a local minimum conformation, which differed from the predominant conformation in the free state by a rotation around the xj/ angle of the o -(l-2) linkage [123]. STD-NMR ex-... 

Transfer NOE experiments have been used to identify a bioactive disaccharide from a library of 15 mono- and disaccharides that bound to Aleuria aurantia agglutinin (179). Another study has described the identification of a silalyl Eewis mimetic (38) that binds to... 

Samples prepared for NMR experiments are in 90% H2O/10%D2O. All 1-D NMR relaxation spectra of the TGF-a/EGFR-ED system were acquired using a Varian Unity-600 NMR spectrometer at pH 6.0 and 298 °K and all 2-D transferred NOE experiments of the desmopressin/NP-II system were performed on a Varian VXR-500 NMR spectrometer at pH 5.7 and 278 °K. The H2O resonance was suppressed by low-power coherent irradiation for 2.0 s prior to each pulse train. 

J. L. Asensio, F. J. Canada, M. Bruix, A. Rodriguez-Romero, and J. Jimenez-Barbero, Studies of the bound conformations of methyl-lactoside and methyl beta-allolactoside to ricin-B chain using transferred NOE experiments in the laboratory and rotating frames, assisted by molecular mechanics and dynamics calculations, Eur. J. Biochem., 230 (1995) 618-630. 

Transferred NOE Experiments (trNOESY) is a quick two-dimensional H NMR experiment that allows the observation of intramolecular proton contacts (<5 A)... 

Siebert, H.-C., Andre, S., Vliegenthart, J.F.G., Gabius, H.-J., and Minch, M.J. 2003. Suitability of binary mixtures of water with aprotic solvents to turn hydroxyl protons of carbohydrate ligands into conformational sensors in NOE and transferred NOE experiments. J. Biomolec. NMR 25 197-215. 

In transferred NOE experiments, NOE effects are generated very efficiently in the bound form and are observed in the free form. The observed NOE indicates that the two protons are close together in the bound form, even though they may be too far to give an NOE in the free form. A recent book is devoted exclusively to the transferred NOE experiment. ... 

The transferred NOE experiment is used to obtain information on the conformation adopted by a small molecule when it is, transiently, part of a high-molecular-weight complex. It requires the small molecule to be in fast exchange between the free and complexed form and that the correlation time of the complex to be much longer than that of the free molecule. 

Transfer NOE Experiments for the Study of Carbohydrate-Protein Interactions... 

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