Transcriptional Profiling

Zeng F, Schultz RM. RNA transcript profiling dnring zygotic gene activation in the preimplantation mouse embryo. Dev Biol 2005 283 40-57. 

The carotenoid isomerase (CRTISO) was the first isomerase associated with the desaturation steps and named at a time when Z-ISO was unknown to exist ise.ws.ieo.iei (and reviewed in references ). In vitro analysis of substrate conversion " and transcript profiling in planta associated CRTISO with the desaturation steps. Isaacson demonstrated that CRTISO is specific for the 7,9 or 7,9- cis bond configuration and is not involved in the isomerization of the l5-l5-cis double bond to the trans conformation. As recently shown, Z-ISO is required for isomerization of the 15-15 cis double bond of phytoene produced in dark-grown tissues as well as in stressed photosynthetic tissues. Therefore, desaturation of phytoene to lycopene involves a two-step desaturation by PDS, followed l5-cis isomerization by Z-ISO, and then each pair of double bonds introduced by ZDS is followed by CRT-ISO-mediated isomerization of the resulting conjugated double bond pair. 

Brocklehurst, K.R. and Morby, A.P., Metal-ion tolerance in Escherichia coli Analysis of transcriptional profiles by gene-array technology, Microbiology, 146 (9), 2277-2282, 2000. 

Advances in genomic technologies such as transcriptional profiling are facilitating the classification of disease at the molecular level. Clinical phenotypes previously... 

Bortoluzzi S et al. The human adult skeletal muscle transcriptional profile reconstructed by a novel computational approach. Genome Res 2000 10 344-349. 

Davies PF et al. A spatial approach to transcriptional profiling mechanotrans-duction and the focal origin of atherosclerosis. TIBTECH 1999 17 347-350. 

MATSUMURA, H., NIRASAWA, S., TERAUCHI, R., Transcript profiling in rice (Oryza sativa L.) seedlings using serial analysis of gene expression (SAGE), Plant J., 1999, 20, 719-726. 

ISHII, M., HASHIMOTO, S.I., TSUTSUMI, S., WADA, Y., MATSUSHIMA, K., KODAMA, T., ABURATANI, H., Direct comparison of genechip and SAGE on the quantitative accuracy in transcript profiling analysis, Genome., 2000, 2, 136-143. 

The Application of Transcriptional Profiling in Model Connectivity and Lead Assessment in Drug Discovery... 

TRANSCRIPTIONAL PROFILING TECHNOLOGY STATE OF THE ART, AND FORESEEABLE FUTURE... 

Table 1 Studies that use transcriptional profiling to compare biological models... 

In summary, the technology and methodology of transcriptional profiling continues to improve. It is foreseeable that transcriptomics will eventually join... 

Figure 17.4. Epigenetics in the nervous system. Regulation occurs in response to synaptic inputs and/or other psychosocial-environmental stimuli. The external stimuli result in changes in the transcriptional profile of the neuron and eventually affects neural function(s). Many disorders of human cognition might involve dysfunction of epigenetic tagging. (See color insert.)...

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