Genomic transcript profiling

Advances in genomic technologies such as transcriptional profiling are facilitating the classification of disease at the molecular level. Clinical phenotypes previously... 

Bortoluzzi S et al. The human adult skeletal muscle transcriptional profile reconstructed by a novel computational approach. Genome Res 2000 10 344-349. 

ISHII, M., HASHIMOTO, S.I., TSUTSUMI, S., WADA, Y., MATSUSHIMA, K., KODAMA, T., ABURATANI, H., Direct comparison of genechip and SAGE on the quantitative accuracy in transcript profiling analysis, Genome., 2000, 2, 136-143. 

Shearstone JR, Allaire NE, Campos-Rivera J et al. Accurate and precise transcriptional profiles from 50 pg of total RNA or 100 flow-sorted primary lymphocytes. Genomics 2006 88 111-121. 

Gunasekera AM, Myrick A, Le Roch K et al (2007) Plasmodium falciparum genome wide perturbations in transcript profiles among mixed stage cultures after chloroquine treatment. Exp Parasitol 117 87-92... 

This chapter will discuss various experimental approaches used to select the relevant species for conduct of toxicology studies for biopharmaceuticals, as well as highlight advances made in scientific approaches and technologies to facilitate this process. Methods discussed include the traditional immunohisto-chemistry and tissue cross-reactivity studies, flow cytometry, protein sequencing, and functional in vitro assays, as well as newer approaches such as utilization of microarray databases for genomic mRNA expression data and use of transcript profiling studies as an adjunct to functional assays, to understand similarity in pharmacological responsiveness between animals and humans. 

Hiiser AT, Chassagnole C, Lindley ND et al (2005) Rational design of a Corynebacterium glutamicum pantothenate production strain and its characterization by metabolic flux analysis and genome-wide transcriptional profiling. Appl Environ Microbiol 71 3255-3268... 

Erdner, D. L., and Anderson, D. M. (2006). Global transcriptional profiling of the toxic dinoflageUate Alexandriumfundyense using massively parallel signature sequencing. BMC Genomics 7(88). 

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