Transcript elongation

Ping YH, Chu CY, Gao H, Jacque JM, Stevenson M, Rana TM (2004) Modulating HlV-1 replication by RNA interference directed against human transcription elongation factor SPT5, Retro-virology 1 46... 

The general topology of rubredoxins is also observed in the general zinc-ribbon motif in RNA polymerases or in transcription factors (59). The first published zinc-ribbon structure was that of the nucleic-acid binding domain of human transcriptional elongation factor TFIIS (PDB file ITFI) 40). These zinc binding domains and rubredoxins... 

Duan, D. R. et al. Inhibition of transcription elongation by the VHL tumor suppressor protein. Science 1995, 269, 1402-6. 

Spt6 H3-H4 Histone-transfer vehicle Transcription-elongation factor (FACT)... 

Orphanides G, LeRoy G, Chang CH, Luse DS, Reinberg D (1998) FACT, a factor that facilitates transcript elongation through nucleosomes. Cell 92 105-116 Orrick LR, Olson MO, Busch H (1973) Comparison of nucleolar proteins of normal rat Uver and Novikoff hepatoma ascites cells by two-dimensional polyacrylamide gel electrophoresis. Proc Natl Acad Sci USA 70 1316-1320... 

Barboric M, PeterUn BM (2005) A new paradigm in eukaryotic biology HIV Tat and the control of transcriptional elongation. PLoS Biol 3 200-203... 

West MJ, Lowe AD, Karn J (2001) Activation of human immunodeficiency virus transcription in T cells revisited NE-kappaB p65 stimulates transcriptional elongation. J Virol 75 8524-8537... 

The most incisive studies of the problem at the molecular level are those from the Felsenfeld laboratory (see, for example. Refs. [75,76]). They have shown that at least under some circumstances, a polymerase can step around a nucleo-some, displacing it in cis, but not causing dissociation. It is not yet clear, however, if this mechanism is physiologically relevant and/or whether it is the only mechanism. There exist results in apparent conflict with this model (i.e.. Ref. [77]). That the in vivo process is certainly more complex than the in vitro models used to date is further indicated by the discovery of elongation factors that markedly increase transcriptional rates and suppress pausing (see, for example, Conaway and Conaway [78]). Thus, the question as to how transcription elongation occurs in a chromatin template remains at least partially unresolved. For a further discussion, see Jackson, this volume, p. 467. 

Wada, T., Orphanides, G., Hasegawa, I., Kim, D.K., Shima, D., Yamaguchi, Y., Fukuda, A., Hisatake, K., Oh, S., Reinberg, D., and Handa, H. (2000) FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH. Mol. Cell 5, 1067-1072. 

S. cerevisiae 1000 molecules/cell. Chdlp is partially redundant with SWI/SNF complex, ISWlp and ISW2p [94,98]. Chdlp may function during transcript elongation [255]. 

---