TR-iBRB cells

Figure 14.2 Phase contrast microscopic images of conditionally immortalized cells forming the inner blood-retinal barrier (A) and time-course of [3H] adenosine uptake by TR-iBRB cells (B). A Conditionally immortalized rat retinal capillary endothelial cell line TR-iBRB, retinal pericyte cell line TR-rPCT and Muller cell line TR-MUL. B The [ H]adenosine (14 nM) uptake was performed at 37°C in the presence (closed circle) or absence (open circle) of Na+.

An example of this analysis method is shown in Figure 14.2B for [3H]adenosine, which is taken up into TR-iBRB cells. [3H]Adenosine uptake increases linearly for at least 5 min in the presence or absence of Na+. Concentration-dependent adenosine uptake is known to take place with... 

Immunohistochemical study ND not determined GLUT1 facilitative glucose transporter MCT1 monocarboxylate transporter CRT creatine transporter LAT1 L-type amino acid transporter TAUT taurine transporter ENT equilibrative nucleoside transporter Oatp organic anion-transporting polypeptide PAH p-aminohippuric acid RUI retinal uptake index TR-iBRB rat retinal capillary endothelial cells. 

K. Hosoya, M. Tomi, S. Ohtsuki, H. Takanaga, M. Ueda, N. Yanai, M. Obinata, and T. Terasaki. Conditionally immortalized retinal capillary endothelial cell lines (TR-iBRB) expressing differentiated endothelial cell functions derived from a transgenic rat. Exp. Eye Res. 72 163-172 (2001). 

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