Tissue using silk scaffolds

Hofmann, S., S. Knecht, R. Langer, D. L. Kaplan, G. Vunjak-Novakovic, H. P. Merkle, and L. Meinel. 2006. Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cells. Tissue Eng 12(10) 2729-38. 

Hofmann, S. et al., CartUage-like tissue engineering using silk scaffolds and mesenchymal stem cells. Tissue Eng, 2006.12(10) 2729-38. 

Marolt, D., Augst, A., Freed, L.E., Vepari, C., Fajardo, R., Patel, N., Gray, M., Farley, M., Kaplan, D., and Vunjak-Novakovic, G. "Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors". Biomaterials 27(36), 6138-6149 (2006). 

Meinel, L., Hofmann, S., Karageorgiou, V., Zichner, L., Langer, R., Kaplan, D., and Vunjak-Novakovic, G. "Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds". Biotechnol. Bioeng. 88(3), 379-391 (2004a). 

Meinel, L. Kargeorgiou, V. Hofmann, S. Fajardo, R. Snyder, B. Li, C. Zichner, L. Langer, R. Vunjak-Novakovic, G. Kaplan, D.L. Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. J. Biomed. Mater. Res. 71 A 25-34 (2004). 

Electrospun SF-based fibers were prepared from aqueous regenerated silkworm silk Bombyx mon)/PEO solutions to be used as scaffolds for tissue engineering (Jin et al. 2004). PEO supplied good mechanical properties to the electrospun fibers. An MeOH posttreatment induced an amorphous to silk p-sheet conformational transition. The electrospun silk membrane was washed with water to remove PEO in order to improve the cell adhesion and proliferation. These silk fibrous membranes were nonimmunogenic, biocompatible, and capable of supporting bone marrow stromal cell (BMSC) attachment. In another work, electrospun wool keratin/silk fibroin (WK/SF) blend nanofibers exhibited higher Cu + adsorption capacity than SF nanofibrous membrane (Ki et al. 2007). 

The ability to pattern 3D biocompatible scaffolds may lead to new fundamental understanding of tissue development and remodeling as well as the formation of multi-cellular aggregates in complex micro-environments that better mimic the targeted tissues of interest. Using direct-write assembly, 3D silk scaffolds for tissue... 

Marolt, D. et al.. Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors. Biomaterials, 2006.27(36) 6138-49. 

PU is a strong, hard-wearing, tear-resistant, flexible, oil-resistant, and blood-compatible polymer. The functional properties of natural macromolecules can be merged with those of synthetic polymers having controllable structures and properties for the production of polymer/protein hybrids. In tissue engineering, silk fibroin/PU blend film can be used as scaffold material for artificial blood vessels [466] (Figure 2.62). Bacterial synthesized cellulose, which was designed... 

Sandra Hofmann, Henri Hagenmuller, Annette M. Koch, Ralph Mu" Her, Gordana Vunjak-Novakovic, David L. Kaplan, Hans P. Meikle and Lorenz Meinel (2007) Control of in vitro tissue-engineered bonelike structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials 28 1152-1162. 

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